曲美他嗪对力竭运动后大鼠心肌组织的保护作用研究  

Protective effect of trimetazidine for the rat myocardial tissue after exhaustion exercise

作　　者：李晓燕[1] 公雪[2] 张红明[1] 胡毓洪[1] 韩淑芳[1] 

机构地区：[1]济南军区总医院心内科,济南250031 [2]山东泰安泰山医学院研究生院,山东泰安271000

出　　处：《解放军医学杂志》2016年第11期896-901,共6页Medical Journal of Chinese People's Liberation Army

基　　金：2014全军后勤科研重大项目子项目(AWS13C008)~~

摘　　要：目的观察曲美他嗪对力竭运动后大鼠心肌组织氧化应激、凋亡水平及能量代谢的影响,探讨曲美他嗪对力竭运动后大鼠心肌组织的保护作用。方法 30只雄性Wistar大鼠随机分为安静对照组(C组)、反复力竭运动组(E组)、反复力竭运动+曲美他嗪组(TE组),每组10只。通过游泳运动建立大鼠力竭运动模型,TE组加用曲美他嗪干预(10mg/kg),各组均于最后一次力竭运动后留取心肌组织。用分光光度法检测大鼠心肌细胞线粒体超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和活性丙二醛(MDA)含量;用RT-PCR和免疫组化法分别检测心肌组织Ba x、Bcl-2和解偶连蛋白2(UCP2)的m RNA和蛋白表达水平;TUNEL法检测心肌细胞凋亡率。结果与C组比较,E组和TE组心肌线粒体SOD及GSH-Px活性均显著降低,其中E组降低更明显;MDA含量均升高,其中E组升高更明显(P<0.01)。与C组比较,E组和TE组Bax表达量均明显升高,Bcl-2表达量均明显降低(P<0.01);与E组比较,TE组Bax表达量明显降低,Bcl-2表达量明显升高(P<0.01)。TUNEL检测结果显示,与C组比较,E组和TE组大鼠心肌细胞凋亡率明显升高(P<0.01);与E组比较,TE组心肌细胞凋亡率明显降低(P<0.01)。与C组比较,E组和TE组UCP2表达量均明显升高(P<0.01);与E组比较,TE组UCP2表达量明显降低(P<0.01)。结论曲美他嗪可减轻力竭运动后大鼠心肌组织损伤,对大鼠心肌具有保护作用。Objective To explore the protective effect of trimetazidine on the myocardial tissue of rats by observing the oxidative stress, apoptosis and energy metabolism in myocardial tissue of rats after exhausted exercise. Methods Thirty healthy adult male Wistar rats were randomly assigned to three groups （10 each）： quiet control group （C）, exhausted exercise group （E） and exhausted exercise plus trimetazidine group （TE）. Exhausted exercise model was established by forcing the rats swim ceaselessly, rats in group TE received hydrochloric acid trimetazidine （10mg/kg） for intervention. The myocardial tissue of rats was collected after the last exhausted exercise. The myocardial tissue of rats was evaluated by HE staining. The concentrations of superoxide dismutase （SOD）, glutathione peroxidase 1 （GSH-Px） and malondialdehyde （MDA） in rats＇ myocardial cell mitochondria were detected by spectrophotometry. RT-PCR and immunohistochemistry were performed to quantify the mRNA and protein levels of Bax, Bcl-2 and uncoupling protein 2 （UCP2） in myocardial tissue of rats. Terminal-deoxynucleoitidyl Transferase Mediated Nick End Labeling （TUNEL） assay was employed to detect the apoptotic rate of myocardial tissue. Results Compared with group C, the SOD and GSH-Px activities in groups E and TE were reduced （P〈0.01）, while the MDA activity was increased （P〈0.01）, and more obvious changes were in group E. The SOD and GSH-Px activities decreased, while the MDA activity increased in group E than in group TE （P〈0.01）. Compared with group C, the apoptotic rate of myocardial tissue increased in groups E and TE （P〈0.01）, and the highest apoptotic rate was in group E （P〈0.01）. Furthermore, the mRNA and protein levels of Bax were significantly elevated in groups E and TE （P〈0.01）, and the highest expression was in group E （P〈0.01）. The mRNA and protein levels of BCL-2 were significantly reduced in groups E and TE （P〈0.01）, and the lowest expression was

关 键 词：曲美他嗪 力竭运动 氧化性应激 细胞凋亡 解偶联蛋白2 

分 类 号：R972.3[医药卫生—药品]