正交设计法优选止咳化痰合剂提取工艺  

Optimization of Extraction Process for Zhike Huatan Mixture by Orthogonal Design

作　　者：毛疆民 刘跃林[2] 龚祖芳 MAO Jiangminl LIU Yueliu Gong Zufang(Rugao People＇s Hospital, Rugao 226500, Chin The Chinese Medicine Hospital of Rugao, Rugao 226500, China Essence Pharmaceutical Group Limited by Share Ltd. Preparation Research Institute, Nantong 226000, China)

机构地区：[1]江苏省如皋市人民医院,江苏如皋226500 [2]江苏省如皋市中医院,江苏如皋226500 [3]精华制药集团股份有限公司制剂研究所,江苏南通226000

出　　处：《中国现代应用药学》2016年第11期1443-1447,共5页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的优选止咳化痰合剂提取工艺。方法采用超声浸泡-水提回流法对药材进行提取。以橙皮苷的提取率、黄芩苷的提取率以及干浸膏得率为综合考察指标,采用正交设计法考察浸泡时间、加水量、提取时间、提取次数4个因素的影响,优选最佳提取工艺,并进行工艺验证。结果最佳工艺为加10倍药材量的水超声浸泡20 min,提取2次,每次80 min。结论验证试验表明,通过正交试验优选的止咳化痰合剂提取工艺稳定可行。OBJECTIVE To optimize the extraction process for Zhike Huatan mixture. METHODS Ultrasonic soakage-reflux extraction method was used for the extraction of medicinal plants. With the overall score of extraction rate of hesperidins and baicalin, and dry ointment yield rate as the observed index, orthogonal design was used to examine four factors including soaking time, additive water amount, reflux time and extraction times in order to optimize the extraction process. And then process certification was carried out. RESULTS The optimum extraction process was as follows： add 10 times amount of water to soak the medicine plants for 20 min using ultrasonic method, then reflux twice, 80 min for each. CONCLUSION The experimental results showed that the extraction process of Zhike Huatan mixture was stable and feasible by orthogonal test.

关 键 词：止咳化痰合剂 橙皮苷 黄芩苷 干浸膏得率 提取工艺 正交设计 

分 类 号：R284[医药卫生—中药学]