平陆百合脱毒苗的生根培养  

Rooting culture of Lilium brownii var.viridulumvirus-free plants

作　　者：任建宏[1] 王鹏丽[1] 殷丽娜[1] 赵娟[1] 

机构地区：[1]山西农业大学农学院,山西太谷030801

出　　处：《山西农业大学学报（自然科学版）》2016年第11期800-804,共5页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：山西省科技攻关项目(20130311014-1);山西农业大学引进人才科研启动项目(2012YJ13)

摘　　要：[目的]建立高效的平陆百合脱毒苗生根系统。[方法]以百合再生小鳞茎为材料,研究了无机盐使用量、不同种类和浓度生长素、不同蔗糖浓度对其生根的影响。[结果]适当降低无机盐用量更适于百合的生根培养;NAA比IBA更利于诱导百合生根,且浓度为0.8mg·L^(-1)时最佳;蔗糖浓度为90g·L^(-1)时百合生根效果最好,过高或过低都会抑制其生根。[结论]平陆百合生根的最佳培养基为:1/2(大量、微量元素)MS+NAA0.8mg·L^(-1)+蔗糖90g·L^(-1),生根率达100%,且生根健壮,适合移栽。[Objective]In order to establish an efficient rooting protocol in vitro of Lilium brownie var.viridulum,[Methods]With the regenerated bulbs of Liliumas materials,the effect of inorganic salt,auxin and sucrose concentration on root formation of Lilium were studied.[Results]Lower inorganic salt concentration was much better for rooting of Lilium;NAA in comparison with IBA,were more suit able for roots formation of Lilium,the optimum concentration was 0.8mg·L^-1,The best induction results were obtained at the concentration of 90g·L^-1.Too high or too low sucrose concentration had some restrainable effect on root formation.[Conclusion]The optimal rooting media of Lilium brownii var.viridulum was 1/2（macroelements、microelements）MS＋0.8 NAA mg·L^-1＋90g·L^-1 sucrose.The rooting rate was 100% and roots were suitable for transplanting.

关 键 词：平陆百合 生长素 生根培养 

分 类 号：S644.1[农业科学—蔬菜学]