西葫芦大孢子离体培养再生植株技术优化研究  

Improving Technology of Megaspore Culture in Vitro and Plantlet Regeneration in Cucurbita pepo

作　　者：胡芳芳[1] 王改改[1] 梁策[1] 刘霞[1] 程永安[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《中国农学通报》2016年第31期29-33,共5页Chinese Agricultural Science Bulletin

基　　金：国家公益性行业(农业)科研专项"南瓜产业技术研究与示范"(201303112);现代农业产业技术体系"国家大宗蔬菜产业技术体系项目"(CARS-25)

摘　　要：西葫芦大孢子离体培养过程中,培养体玻璃化现象是再生植株形成的主要障碍之一。为保障西葫芦大孢子培养过程中胚状体的正常产生,减少培养体玻璃化现象对再生植株形成的影响,提高试管苗成活率。试验通过4种培养基诱导胚状体形成筛选优良诱导培养基,通过对组织培养过程中环境温度、湿度、GA浓度等因子调整来降低培养体玻璃化苗的发生。结果表明,4种培养基均能不同程度诱导胚状体形成,其中D号B5+40 g/L蔗糖+8 g/L琼脂+0.5 mg/L NAA+0.05 mg/L TDZ+30 mg/L Ag NO3培养基最佳(诱导出胚达96%),且直接生成76%不定芽;在培养温度为25℃,湿度85%,生根培养基GA浓度0.5 mg/L条件下西葫芦大孢子培养体玻璃化苗发生率最低。试验在前人基础上完善、优化、建立了最佳的西葫芦未受精子房再生植株技术体系,为后续西葫芦的研究提供参考。The vitrification phenomenon of culturing body is one of the main obstacles to regenerated plantletformation in the process of in vitro culture of megaspore zucchini（Cucurbita pepo）. The aim of the research is toguarantee normal production of the embryoid, minimize the impact of vitrification phenomenon on plantletregeneration from megaspore and enhance the survival rate of test-tube plantlets. In the experiment, 4 kinds ofmedia-induced somatic embryogenesis were selected to induce the formation of excellent induction medium.And the environmental temperature, humidity and concentration of GA were adjusted to reduce the occurrenceof vitrification shoots of culturing body. The results showed that the four kinds of different levels of culturemedium could induce the formation of embryoid bodies. One of the best was No. D： B5＋40g/L Suc＋8g/LAgar＋0.5mg/L NAA＋ 0.05 mg/L TDZ＋30 mg/L AgNO_3. Meanwhile, embryo induction was up to 96% and 76% adventitious bud was generated directly. In the condition of 25℃, 85% humidity, and 0.5% rootingmedium GA concentration, the vitrification shoots rate of culturing body was the lowest. On the basis ofprevious researches, the experiment optimized and established the best zucchini plant regeneration unfertilizedroom technology system, provided a reference for further study on zucchini.

关 键 词：西葫芦 优化 胚状体 玻璃化现象 

分 类 号：S642.6[农业科学—蔬菜学]