苹果花药石蜡切片制片技术改良及其解剖学观察  

Improvement of Paraffin Section Methods and Structural Observation of Apple Anther

作　　者：冯丽云[1,2] 张春芬[3] 聂园军[4] 邓舒[3] 温鑫[1,2] 肖蓉[3] 李倩[2] 任莹 罗慧珍[1,2] 曹秋芬[1,2] 

机构地区：[1]山西大学生物工程学院,太原030006 [2]山西省农业科学院生物技术研究中心,太原030031 [3]山西省农业科学院果树研究所,山西太谷030815 [4]山西省农业科学院农业资源与经济研究所,太原030006

出　　处：《中国农学通报》2016年第31期62-67,共6页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金"苹果纯合基因型种质群体的创建及小孢子诱导产生胚状体机理的研究"(31372033);国家科技基础性工作专项"我国西部地区落叶果树农家品种资源调查与收集"(2012 FY110100-5);山西省农业科学院博士基金"‘嘎啦’和‘富士’苹果花药培养纯合基因型种质群体的建立"(YBSJJ1515)

摘　　要：石蜡切片是常规制片技术中应用最普遍的方法,主要用于观察组织细胞结构。本研究以苹果花药作为实验材料,通过改良脱水、染色等制片环节,即增加脱水时乙醇的浓度梯度,以及用埃里希氏(Ehrlich)苏木精染色15 min,得到了染色更均匀、细胞完整度更高的玻片标本。实验结果表明,用改良后的制片方法得到切片组织更完整,更能清晰地辨认出花粉细胞的结构,利于观察。Paraffin section, the most common method in conventional sectioning, is often used to observe thecellular structure. In this study, the apple anther was used as the experimental material, the dehydration,dyeing and other slice making steps were modified. The extra gradients of ethanol concentration were addedand the samples were stained with Ehrlich hematoxylin for 15 min. The dyeing of sample slices was morehomogeneous and more complete cell structures were obtained. The results showed that the modified paraffinsection method was more suitable for apple anther.

关 键 词：苹果花药 石蜡切片 染色 观察 

分 类 号：S330[农业科学—作物遗传育种]