氟硅唑·丙环唑混合药剂高效液相色谱分析  

Analysis of Flusilazole·Propiconazole by HPLC

作　　者：陈章明[1] 杨石有[1,2] 张蕊[1] 张贺[3] 

机构地区：[1]海南大学应用科技学院,海南儋州571737 [2]河北农业大学植物保护学院,河北保定071001 [3]中国热带农业科学院环境与植物保护研究所,海口571101

出　　处：《农药》2016年第11期814-816,共3页Agrochemicals

基　　金：海南省自然科学基金项目(20163063);公益性行业(农业)科研专项项目(201203092-2);海南大学应用科技学院院基金(Hyk-1514)

摘　　要：[目的]建立一种高效液相色谱法测定氟硅唑·丙环唑混合药剂有效成分含量的定量分析方法。[方法]采用高效液相色谱法,以甲醇和水(体积比85∶15)为流动相,流速为1 m L/min,以220 nm为检测波长,对混合样中氟硅唑和丙环唑进行液相色谱分离测定。[结果]氟硅唑和丙环唑保留时间分别为4.627、5.640 min,线性相关系数分别为0.9999和0.9983,标准偏差分别为0.0873、0.0707,变异系数分别为0.3363%、0.0945%;平均回收率分别为98.80%、97.34%。[结论]该方法操作简便,分离效果好,精密度与准确度高,可同时测定氟硅唑和丙环唑含量。[Aims] The HPLC method for the determination of active ingredients in flusilazole .propiconazole was established. [Methods] The separation and determination of flusilazole and propiconazole in the samples were conducted by HPLC with the mixture of methanol and water （85：15, by vol） as mobile phase at the flow rate of 1 mL/min at 220 nm. [Results] The retention times of flusilazole and propiconazole were 4.627 and 5.640 min, the linear correlation coefficients for flusilazole and propiconazole were 0.9999 and 0.9983, the standard deviations were 0.0873 and 0.0707, the variation coefficients were 0.3363 and 0.0945%, and the average recoveries were 98.80 and 97.34%, respectively. [Conclusions] The method has the advantages of simple, convenient, good separating effect, high precision and accuracy, which can be used in simultaneous determination of flusilazole and propiconazole.

关 键 词：氟硅唑 丙环唑 高效液相色谱 分析 

分 类 号：TQ455.4[化学工程—农药化工] O657.72[理学—分析化学]