苏云金杆菌及其毒蛋白对土耳其斯坦叶螨不同螨态的毒力测定  

Toxicities of Bacillus thuringiensis and Toxic Protein to Different Developmental Stages of Tetranychus turkestani

作　　者：包建红[1] 王小军[1] 张燕娜[1] 祝儒伟 赵伊英[1] 

机构地区：[1]石河子大学农学院,新疆石河子832000

出　　处：《农药》2016年第11期847-850,共4页Agrochemicals

基　　金：国家自然科学基金(31560532)

摘　　要：[目的]选取苏云金芽孢杆菌及其2种毒蛋白(Cry1Ac活化毒素/原毒素、Cry2Ab活化毒素/原毒素)对土耳其斯坦叶螨不同发育阶段进行毒力测定,为新型生物杀虫剂在土耳其斯坦叶螨生物防治及应用方面提供理论依据。[方法]采用玻片浸渍法和叶片浸渍法。[结果]2种活化毒素对若螨和雌成螨有较高毒力,其中Cry2Ab最高,LC_(50)值分别为30.68、144.58 mg/L,其次是Cry1Ac,LC50值为79.86、187.29 mg/L。但是对卵的活性明显偏低。苏云金杆菌制剂及2种原毒素对土耳其斯坦叶螨3种螨态毒力均较低。[结论]2种活化毒素对若螨和雌成螨有毒力,可以用于土耳其斯坦叶螨的生物防治。[Aims] The study aims to test the toxicities of Bacillus thuringiensis and two kinds of toxicity protein （CrylAc activated toxin/protoxin and Cry2Ab activated toxin / protoxin） on different developmental stages of T. turkestani and provide theoretical basis for biological control and practical application of biological pesticides against T. turkestani. [Methods] Toxicity was tested by using slide-dipping method and leaf-dipping method. [Results] The result showed that 2 kinds of activated toxin had high toxicity, and the Cry2Ab activated toxin had the highest toxicity to nymphs and adults, LC50 was 30.68 and 144.58 mg/L; followed by the CrylAc activated toxinwith LC50 of 79.86 and 187.29 mg/L. However, they both had low toxicity to eggs. Additionally, Bacillus thuringiensis and 2 kinds of protoxin had low toxicity to 3 different stages. [Conclusions] The 2 kinds of activated toxins had higher toxicity to nymphs and adults, which suggested that they could be used in the biological control of T. turkestani.

关 键 词：苏云金杆菌 Cry毒蛋白 土耳其斯坦叶螨 毒力 

分 类 号：S482.39[农业科学—农药学] S433.7[农业科学—植物保护]