宇香色酮和槲皮素体外抑制水痘-带状疱疹病毒作用机制比较  

In vitro antiviral mechanisms of eugeniin and quercetin against varicella-zoster virus

作　　者：李晓霞[1] 廉成翔 李晓杰[1] 王官清[2,3] 井上直树 

机构地区：[1]上海交通大学附属第一人民医院感染性疾病科,201620 [2]上海交通大学附属第一人民医院皮肤科,201620 [3]厦门大学附属中山医院皮肤科,361004 [4]日本岐阜药科大学微生物与免疫学系,502-8585

出　　处：《中华微生物学和免疫学杂志》2016年第10期727-733,共7页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金资助项目（30671884,30840070）;厦门市科技计划资助项目（3502220089030）

摘　　要：目的：应用基于水痘-带状疱疹病毒（VZV）报告细胞系MV9G建立的报告细胞法比较宇香色酮和槲皮素体外抑制VZV的作用机制。方法测定宇香色酮、丁子香酚、桑色素、姜黄素、杨梅黄酮、槲皮素等中药或植物成分体外抑制 VZV 的选择指数（SIs），进一步应用无细胞病毒（CFVs）直接感染法和带细胞病毒（CAVs）共培养法分析SIs较高的化合物在直接灭活CFVs、抑制CFVs黏附和穿透、抑制CAVs细胞内复制、抑制病毒即刻早期基因62（IE62）转录和表达等方面的作用。结果宇香色酮和槲皮素SIs相对较高，分别为5.82和8.97。宇香色酮部分灭活CFVs并抑制CFVs黏附和穿透MV9G细胞，槲皮素无此作用。宇香色酮和槲皮素可逆性抑制CAVs细胞内复制以及病毒IE62基因转录及IE62抗原表达，但宇香色酮需在感染12 h内加入。结论宇香色酮和槲皮素体外抑制VZV作用机制有差异，抑制病毒IE62基因转录和表达是其共同机制之一。Objective To comparatively analyze the in vitro antiviral mechanism（ s） of eugeniin and quercetin against varicella-zoster virus （ VZV） by using a novel antiviral assay based upon a reporter cell line （MV9G cells） for VZV. Methods Selection indexes （SIs） of potential antiviral compounds extracted from Chinese herbs or plants including eugeniin, eugenol, morin, curcumin, myricetin and quercetin for in vitro inhibition of VZV were calculated. The compounds with relatively higher SIs were screened out for fur-ther investigation of their in vitro inhibitory mechanisms with a cell-free virus （ CFVs） direct-infection assay and a cell-associated virus （CAVs） co-culture assay established with MV9G cells in our previous study. The inhibitory mechanisms analyzed in this study included direct inactivation of CFVs, inhibition of the adhesion and/or penetration capabilities of CFVs to MV9G cells, inhibition of the intracellular replication of CAVs and inhibition of the transcription and / or expression of viral immediate early gene 62 （ IE62 ） . Results Among the tested compounds, eugeniin and quercetin showed relatively higher SIs of 5. 82 and 8. 97, respec-tively. Eugeniin rather than quercetin directly but partly inactivated CFVs and inhibited their attachment to and penetration into MV9G cells in a concentration-dependent manner. Both eugeniin and quercetin revers-ibly inhibited the intracellular replication of CAVs and the transcription and expression of viral IE62 gene, for which eugeniin needed to be added within 12 hours after infection. Conclusion Eugeniin and quercetin had different in vitro inhibitory mechanisms against VZV, but inhibiting the transcription and expression of viral IE62 gene was a common mechanism shared by both of them.

关 键 词：水痘-带状疱疹病毒 即刻早期基因62 抗病毒 宇香色酮 槲皮素 

分 类 号：R285[医药卫生—中药学]