蛋黄卵磷脂中鞘磷脂的分离与结构鉴定  

Isolation and Identification of Sphingomyelins in Egg Yolk Lecithin

作　　者：李继荣[1] 唐顺之[1] 牟肖男 关伟键 蔡鸿飞[1] 杨玉琼[1] 许文东[1] Li Jirong Tang Shunzhi Mu Xiaonan Guan Weijian Cai Hongfei Yang Yuqiong Xu Wendong(National Engineering Research Center for Modernization of Extraction and Separation Process of TCM/Guangzhou Hanfang Pharmaceutical Co., Ltd., Guangzhou 510240, China)

机构地区：[1]中药提取分离过程现代化国家工程研究中心/广州白云山汉方现代药业有限公司,广东广州510240

出　　处：《广东化工》2016年第22期206-207,共2页Guangdong Chemical Industry

基　　金：广东省药用脂质重点实验室2016B030302003;广东省高层次人才特殊支持计划2014TQ01R131;广州市产学研协同创新联盟专题201605131249066;广州市珠江科技新星专题201506010088

摘　　要：目的:通过柱层析与高效液相色谱方法对蛋黄卵磷脂中的鞘磷脂进行制备分离,采用ESI质谱、GC-MS、核磁共振方法对鞘磷脂进行结构鉴定。结论:从蛋黄卵磷脂中分离鉴定了5种鞘磷脂,分别是N-棕榈酰-3-磷酸胆碱酰鞘氨醇、N-亚油酰-3-磷酸胆碱酰鞘氨醇、N-油酰-3-磷酸胆碱酰鞘氨醇、N-硬脂酰-3-磷酸胆碱酰鞘氨醇以及N-花生四烯酰-3-磷酸胆碱酰鞘氨醇。蛋黄卵磷脂中鞘磷脂成分的全面分析为蛋黄卵磷脂的产品质量研究以及鞘磷脂在医药方面的药理研究与应用等方面提供物质结构参考。Sphingomyelins were separated and purified by silica gel and high performance liquid chromatography from egg yolk lecithin. Their structures were determined by ESI mass spectrometry, GC-MS and NMR. Five compounds were identified as N-palmitoyl-3-sphingosylphosphorylcholine, N-linoleoyl-3 -sphingosylphosphorylcholine, N-oleoyl-3-sphingosylphosphorylcholine, N-stearoyl-3-sphingosylphosphorylcholine, N-arachidonoyl-3-sphingosylphosphorylcholine. Comprehensive analysis of sphingornyelin components in the egg yolk lecithin provides material structure references for the quality and pharmacological research of egg yolk lecithin products.

关 键 词：蛋黄卵磷脂 鞘磷脂 结构鉴定 

分 类 号：TQ28[化学工程—有机化工]