短期喂食转基因大豆对雄性大鼠睾丸StAR表达的影响  

Effects of short term feeding transgenic soybean on the expression of StAR in rat testis

作　　者：史宗勇[1] 许冬梅[1] 路超[1] 袁建琴[1] SHI Zong-yong XU Dong-mei LU Chao YUAN Jian-qing(College of Life Science, Shanxi Agricultural University, Taigu , Shanxi 030801, China)

机构地区：[1]山西农业大学生命科学学院,山西太谷030801

出　　处：《食品与机械》2016年第10期12-16,共5页Food and Machinery

基　　金：山西省自然科学基金(编号:2013011028-2);山西省科技攻关基金(编号:20140311025-3)

摘　　要：探讨转基因大豆对雄性大鼠睾丸类固醇激素合成急性调节蛋白(steroidogenic acute regulatory protein,StAR)mRNA水平和蛋白水平表达的影响,为转基因大豆的安全性提供科学依据。将30只健康雄性离乳SD大鼠随机分成两组,设GM组喂食含转CP4-epsps基因大豆饲料,CK组喂食含非转基因受体大豆(Soybean A5403)饲料,喂食试验饲料30,60,90d后,各组随机处死3只大鼠,针对雄性主要生殖器官睾丸、附睾进行脏器系数分析;运用RT-PCR技术检测大鼠睾丸StAR基因表达量;蛋白酶联免疫反应分析大鼠睾丸StAR蛋白含量。喂食30,60,90d后,解剖观察大鼠,与CK组相比GM组大鼠睾丸、附睾形态、颜色正常,大小、器官间比例协调,大鼠睾丸系数无显著性差异,附睾系数除30d处理组(P<0.05)差异显著外,其它各处理组间t检验P值均大于0.05,无显著性差异;GM组与CK组大鼠相比,StAR基因相对表达量、蛋白含量相同处理时间组间差异不显著。短期喂食大鼠转CP4-epsps基因大豆,雄性大鼠主要生殖器官睾丸、附睾生长发育正常,喂食转基因大豆对大鼠睾丸中StAR基因表达量、StAR蛋白含量无明显影响。The present study was conducted to discuss the effects on genetically modified soybeans on the level of mRNA and protein in StAR gene in male rat, and aimed to provide a scientific basis for the security of transgenic soybeans. Thirty male SD rats were randomly divided into GM group and CK group. The GM group were fed with transgenic Roundup Ready soybean containing CP4-EPSPS gene, and the CK group were fed with non-transgenic soybean. Three rats ofevery group were killed at 30 d, 60 d and 90 d, respectively. Viscera index （testes and epididymides） was calculated. RT-PCR was used to measure the expression of StAR gene, and EI.ISA was used to analy sis the expression of StAR protein. The morphology and color of testes and epididymides of the GM group and CK group were ob- served. No significant differences were found in size and testis coeffi- cient. Epididymis coefficient were no significant difference（P〉0.05） except 30 d group. The average expression of StAR gene and protein in GM and CK group （30 d 60 d and 90 d） was detected, and no sig nificant differences between the two groups at the same time were found. The results suggested that feeding transgenic soybean for 30 d showed no damages to occurrence of testes and epididymides in rat, and the expression of StAR gene and protein were no difference either.

关 键 词：转基因大豆 大鼠 脏器系数 STAR 

分 类 号：S565.1[农业科学—作物学]