羟基红花黄色素A抑制脂多糖诱导的急性肺损伤小鼠炎症因子表达的研究  

Hydroxysafflow Yellow A Inhibits Inflammatory Factors Expression in Acute Lung Injury Mice Induced by Lipopolysaccharide

作　　者：宋丽娟[1] 朱煜[2,3] 金鸣[2] Song Lijuan Zhu Yu Jin Ming(Clinical Institute of Traditional Chinese Medicine, Shanxi University of Traditional Chinese Medicine, Taiyuan , Shanxi, 030619 , China)

机构地区：[1]山西中医学院中医临床学院 [2]首都医科大学附属安贞医院北京市心肺血管疾病研究所药理研究室 [3]解放军63628部队卫生队药剂科

出　　处：《中国呼吸与危重监护杂志》2016年第6期577-582,共6页Chinese Journal of Respiratory and Critical Care Medicine

基　　金：北京市自然科学基金(编号:7102025);山西省卫生和计划生育委员会科研课题(编号:2015104);山西中医学院博士科研启动基金(编号:2014BK11)

摘　　要：目的探讨羟基红花黄色素A(HSYA)是否可抑制肺部炎症信号转导通路的相关环节。方法以脂多糖(LPS)腹腔注射法建立急性肺损伤模型。将84只雄性昆明鼠随机分为7组,每组12只,包括空白组、LPS组、地塞米松(DXM)组(LPS+DXM)、HSYA低剂量组(LPS+HSYA6 mg/kg)、HSYA中剂量组(LPS+HSYA 15 mg/kg)、HSYA高剂量组(LPS+HSYA 37.5 mg/kg),以及HSYA对照组(生理盐水+HSYA 37.5 mg/kg)。采用ELISA法测小鼠血清中肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)及IL-6水平;RT-q PCR法检测肺组织Toll样受体4(TLR4)mRNA的表达,Western blot法检测肺组织TLR4蛋白的表达。结果 HSYA在浓度分别为6、15、37.5 mg/kg时均可抑制脂多糖诱导的急性肺损伤小鼠肺组织中TLR4 mRNA和蛋白的表达,以及外周血中TNF-α、IL-1β及IL-6蛋白的表达,且抑制效应随着HSYA剂量升高而增强。DXM抑制效应强于HSYA。结论HSYA对LPS诱导的小鼠急性肺损伤中TLR4、TNF-α、IL-1β及IL-6表达升高有抑制作用,且呈现一定的量效趋势,但其抑制效应弱于DXM。Objective To study the effects of hydroxysafflow yellow A（ HSYA） in inhibiting inflammatory signal transduction in lungs of acute lung injury mice induced by lipopolysaccharide（ LPS）.Methods Eighty-four male Kunming mice were randomly divided into 7 groups,ie. a sham group,a LPS group,a LPS ＋ 3 mg / kg dexamethason（ DXM） group,a LPS ＋ 6 mg / kg HSYA group,a LPS ＋ 15 mg / kg HSYA group,a LPS ＋ 37. 5 mg / kg HSYA group,and a saline ＋ 37. 5 mg / kg HSYA group（ n = 12 in each group）. The mice were intraperitoneally pretreated with normal saline or DXM or HSYA 0. 5 hour prior to intravenous adminstration of LPS. TNF-α,IL-1β and IL-6 levels in mice serum were measured by ELISA and the mRNA and protein levels of TLR4 in mice lungs were assessed by RT-q PCR and Western blot,respectively. Results After being treated with HSYA in doses of 6 mg / kg,15 mg / kg,and 37. 5 mg / kg,the increased expression levels of TLR4 mRNA and protein induced by LPS were significantly inhibited,as well as the increased expression levels of TNF-α,IL-1β and IL-6. The inhibitoty effect enhanced with the doses of HSYA. DXM could inhibit more significantly the increased expression levels of all the indexes.Conclusion HSYA can inhibit inflammatory signal transduction in acute lung injury mice induced by LPS in a dose-dependent manner,but is less effective than DXM.

关 键 词：羟基红花黄色素A 急性肺损伤 脂多糖 

分 类 号：R563.8[医药卫生—呼吸系统]