采用正交t值法优选治疗急性痛风性关节炎的中药有效成分复方配伍  

Compound Optimization of Effective Components of Traditional Chinese Medicine in Treatment of Acute Gouty Arthritis by Orthogonal T Value Method

作　　者：路占忠 李振彬[1] 马旭[1] 宋哲[1] 

机构地区：[1]解放军白求恩国际和平医院中西医结合-风湿免疫科,石家庄050082 [2]石家庄市第三医院风湿免疫科,石家庄050011

出　　处：《解放军医药杂志》2016年第11期33-36,41,共5页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：河北省科技计划项目(13277704D);河北省中医药管理局科研计划项目(2014069)

摘　　要：目的针对大鼠急性痛风性关节炎（AGA）模型筛选最优抗炎配伍植物中药有效成分组合复方。方法将SD雄性大鼠65只随机分成12组,并设置空白对照组,每组5只。按照正交t值表分配白芍总苷（TGP）、三七总皂苷（PNS）、青藤碱（SN）、雷公藤多苷（TG）及人参皂苷（GS）5种中药有效成分配伍组合,各组每只1.5 ml/d等体积灌胃给药。给药第6天第1~12组大鼠左踝关节腔注射PBS配制的微晶尿酸钠结晶（25 mg/ml）100μl进行造模,造模前及造模后8 h测量踝关节周径计算其肿胀度。结果模型组与对照组相比造模8 h后肿胀度均有显著增加（P〈0.01）。经正交t值法肿胀度筛选,TGP、PNS、SN在复方组合中药效突出,其中SN最有意义（P〈0.01）;而TGP和PNS在复方中也具有统计学意义（P〈0.05）。结论 SN、TGP、PNS在5种有效成分组成的复方中发挥主导抗炎效应。Objective To select the optimal anti-inflammatory compound of effective ingredients of traditional Chinese medicine in treatment of rats with acute gouty arthritis（AGA）.Methods A total of 65 SD male rats were randomly divided into 12 groups and a control group（n = 5 for each group）.Effective ingredient combinations of Tripterygium Glycosides（TG）,Ginsenosids（GS）,Total Glucosides of Paeony（TGP）,Panax Notoginseng Saponins（PNS） and Sinomenine（SN） were distributed according to orthogonal t value table,and each group was performed intragastric administration by 1.5 ml per rat with equal volume daily.On the 6th d of drug intervention,the 12 groups were injected with 100 μl crystallite sodium urate（25 mg / ml） prepared by phosphate buffer solution（PBS） into left ankle joint cavity for model establishment,and the AGA models were established.Circumference and swelling degree of the ankle joint were measured and calculated before and 8 h after model establishment.Results Compared with that in control group,the swelling degrees of model groups were significantly increased 8 h after model establishment（P〈0.01）.The screening of orthogonal t value method for swelling degree showed that effects of TGP,PNS and SN in compound combinations were better,and SN was the most significant one（P〈0.01）,and TGP and PNS in the compounds were also statistically significant（P〈0.05）.Conclusion SN,TGP and PNS play leading anti-inflammatory effect in the compound by 5 effective components.

关 键 词：急性痛风性关节炎 有效成分配伍 正交T值法 大鼠 SD 抗炎效应 

分 类 号：R589.7[医药卫生—内分泌]