过氧化物还原酶Ⅰ、Ⅱ在先兆流产患者子宫内膜中的表达及意义  

Clinical expression and significance of Prx Ⅰand Prx Ⅱ for patients with threatened abortion

作　　者：余碧雅 林春燕 钟秀妹 余进胜 

机构地区：[1]广东省惠州市惠东县人民医院妇产科,广东惠东516300

出　　处：《中国当代医药》2016年第32期83-86,共4页China Modern Medicine

基　　金：广东省惠州市科技项目(2014Y242)

摘　　要：目的 探讨过氧化物还原酶Ⅰ（PrxⅠ）、Ⅱ在先兆早期流产患者子宫内膜中的表达及意义,从氧化应激方面探讨先兆早期流产的发病机制。方法 选取2014年4月～2016年4月就诊于惠州市惠东县人民医院计划生育门诊的流产患者60例,其中符合早期自然流产患者30例（实验组）,自愿人工流产终止妊娠者30例（对照组）。采集两组患者的流产蜕膜标本,采用免疫组化及免疫蛋白印记法检测组织中PrxⅠ、Ⅱ蛋白含量,比较两种蛋白在自然流产及正常早孕患者中的表达差异。结果 实验组患者的蜕膜组织中PrxⅠ蛋白定性表达阳性率（40.0%）显著低于对照组（90.0%）,其中实验组＋＋阳性率为6.7%、＋＋＋阳性率为3.3%,对照组＋＋阳性率为53.3%,＋＋＋阳性率为16.7%,差异有统计学意义（P〈0.05）。实验组患者的蜕膜组织中PrxⅡ白定性表达阳性率（40.0%）显著低于对照组（93.3%）,其中实验组＋＋阳性率为13.3%,＋＋＋阳性率为3.3%,对照组＋＋阳性率为56.7%,＋＋＋阳性率为20.0%,差异均有统计学意义（P〈0.05）。实验组患者的蜕膜组织中PrxⅠ及PrxⅡ蛋白表达量（0.217±0.018、0.224±0.021）均明显低于对照组（1.174±0.114、1.184±0.181）,差异有统计学意义（t=31.942、29.481,均P〈0.05）。Pearson相关性分析结果显示,实验组滋养细胞中PrxⅠ和PrxⅡ在实验组蜕膜组织中的表达量呈正相关（r=0.782,P〈0.05）。结论 PrxⅠ和PrxⅡ两种蛋白表达具有相关性,均具有较强的抗氧化应激作用,该蛋白的大量丢失可能是自然先兆流产发生的主要机制之一。Objective To study the clinical expression of PeroxiredoxinⅠ（Prx Ⅰ） and PeroxiredoxinⅡ（Prx Ⅱ） for patients with threatened abortion and explore the pathogenesis of threatened abortion from the aspects of oxidative stresss.Methods Sixty patients with threatened abortion treated from April 2014 to April 2016 in the Huidong County People′s Hospital of Huizhou City were selected.30 cases of patients adopting the spontaneous abortion were selected as experimental group;30 cases of adopting the induced abortion were selected as control group.The specimen of decidua was collected.Through the immunohistochemical method and western blot,the content of Prx Ⅰand Prx Ⅱ for spontaneous abortion and early pregnancy group was detected and compared.Results The positive rate of Prx Ⅰin the decidual tissues of the experimental group（40.0%） was lower than that of the control group（90.0%）;the positive rate of（＋＋） and（＋＋＋） in experimental group（6.7%,3.3%） was lower than those of the control group（53.3%,16.7%）,the difference was statistically significant（P〈0.05）.The positive rate of Prx Ⅱ in the decidual tissues of the experimental group（40.0%） was lower than that of the control group（93.3%）;the positive rate of Prx Ⅱ in the decidual tissues of the experimental group（40.0%） was lower than that of the control group（90.0%）;the positive rate of（＋＋） and（＋＋＋） in the experimental group（13.3%,3.3%） was lower than those of the control group（56.7%,20.0%）,the difference was statistically significant（P〈0.05）;the expression of Prx Ⅰ and Prx Ⅱ protein in the decidual tissues of the experimental group（0.217±0.018,0.224±0.021） was lower than that of the control group（1.174±0.114,1.184±0.181）,the differences were statistically significant（t=31.942,29.481,all P〈0.05）.Pearson correlation analysis showed that,the expression of PrxⅠ protein was positively correlated with Prx Ⅱprotein in the decidual tissues of the

关 键 词：过氧化物还原酶Ⅰ 过氧化物还原酶Ⅱ 先兆流产 子宫内膜 

分 类 号：R715.2[医药卫生—妇产科学]