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作 者:潘晓琴[1] 谭传梅[1] 唐红艳[1] 丁异熠[1]
机构地区:[1]湖南省常德市第一人民医院儿科,湖南常德415003
出 处:《中国当代医药》2016年第32期96-98,共3页China Modern Medicine
摘 要:目的 探讨Xpert MTB/RIF检测法在儿童肺泡灌洗液结核分枝杆菌检测中的应用价值。方法 选择2014年4月~2016年4月于常德市第一人民医院儿科住院的162例疑似肺结核患儿为研究对象,同时采用直接涂片抗酸染色法、离心涂片抗酸染色法和Xpert MTB/RIF检测法检测患儿肺泡灌洗液中抗酸杆菌,比较三种方法抗酸杆菌的阳性率。结果 直接涂片抗酸染色法、离心涂片抗酸染色法和Xpert MTB/RIF检测法的阳性率分别为14.8%、29.6%、78.3%,离心涂片抗酸染色法阳性率高于直接涂片抗酸染色法,差异有统计学意义(配对χ^2=4.56,P〈0.05);Xpert MTB/RIF检测法高于离心涂片法,差异有统计学意义(配对χ^2=16.32,P〈0.01)。结论 三种方法中,Xpert MTB/RIF检测法是一种快速、简便、阳性率高的检测技术,值得临床推广应用。Objective To explore the application value of Xpert MTB/RIF in testing of mycobacterium tuberculosis in bronchoalveolar lavage fluid.Methods From April 2014 to April 2016,162 children with suspected tuberculosis in Department of Pediatrics of the First People′s Hospital of Changde were selected as the research objects,at the same time direct smear acid-fast stain,centrifugation-smear acid-fast stain and MTB/RIF test were used to detect the acid-fast bacillus in alveolar lavage fluid,and the positive rate of acid-fast bacillus of three methods was compared.Results Positive rate of direct smear anti-acid stain,centrifugation-smear acid-fast stain and Xpert MTB/RIF test were 14.8%,29.6%,78.3% respectively.The positive rate of the centrifugation-smear acid-fast stain was higher than that of the direct smear anti-acid stain the positive rate,the difference was statistically significant(χ^2=4.56,P〈0.05);compared with centrifugation-smear anti-acid stain method,the positive rate of the Xpert MTB/RIF test was higher than that of the centrifugation-smear acid-fast stain,the difference was statistically significant(χ^2=16.32,P〈0.01).Conclusion Among the three methods,Xpert MTB/RIF test is a technology,that rapid,convenient,and with high positive rate,it is worthy of clinical application.
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