脑脊液免疫球蛋白和寡克隆区带检测在小儿病毒性脑炎中的临床意义  

Clinical significance of the detection in immunoglobulin in CSF and oligoclone IgG band children with viral encephalitis

作　　者：翁翔 兰帝仕 

机构地区：[1]福建省福州儿童医院,福建福州350004

出　　处：《中国当代医药》2016年第32期99-101,共3页China Modern Medicine

摘　　要：目的 研究脑脊液免疫球蛋白和寡克隆区带检测在小儿病毒性脑炎诊断中的临床意义。方法 选取2014年6月～2015年6月于福州儿童医院住院治疗的40例患儿为研究对象,分为病毒性脑炎组（25例）和非病毒性脑炎组（15例）。检测并比较两组患儿脑脊液白蛋白（Alb）、免疫球蛋白（Ig G、Ig A、Ig M）的表达水平,并经酶放大免疫固定电泳技术证实和分辨寡克隆区带中的免疫球蛋白,比较两组患儿寡克隆区带Ig表达情况。结果 病毒性脑炎组患儿脑脊液中Alb、Ig G、Ig A、Ig M分别为（0.18±0.08）g/L、（35.55±18.65）mg/L、（2.46±1.64）mg/L、（0.98±0.32）mg/L;非病毒性脑炎组脑脊液的Alb、Ig G、Ig A、Ig M分别为（0.16±0.08）g/L、（10.45±2.62）mg/L、（0.54±0.38）mg/L、（0.59±0.15）mg/L。两组患儿脑脊液中Ig G和Ig A水平比较差异有统计学意义（P〈0.05）,而Alb和Ig M水平比较差异无统计学意义（P〉0.05）。病毒性脑炎组患儿脑脊液寡克隆区带免疫球蛋白阳性3例,阳性率为12%,非病毒性脑炎组脑脊液寡克隆阳性率为0%,两组比较差异无统计学意义（P〉0.05）。结论 脑脊液免疫球蛋白Ig G和Ig A含量增高对诊断小儿病毒性脑炎有一定的参考价值,值得临床应用。寡克隆区带检测对小儿病毒性脑炎的诊断有一定的提示作用,尚需更多的样本进一步研究。Objective To study the clinical application value of cerebrospinal fluid immunoglobulin and oligoclonal zone detection in the diagnosis of infantile encephalitis viral infection.Methods Forty children from June 2014 to June 2015 in Fuzhou Children′s Hospital of Fujian Province were selected as the study objects,and they were divided into the viral encephalitis group（25 cases） and non-encephalitis group（15 cases）.The expression of albumin（Alb）,immunoglobulin（Ig G,Ig A,Ig M）in cerebrospinal fluid（CSF） of the two groups were detected and compared;the immune globulin（Ig） in the oligoclonal zone（OCB） was detected by enzyme immune fixed electrophoresi,and the the expression of Ig in the OCB of the two groups were compared.Results The Alb,Ig G,Ig A and Ig M in CSF of the encephalitis group were（0.18±0.08） g/L,（35.55±18.65） mg/L,（2.46±1.64） mg/L,（0.98±0.32） mg/L respectively,and the non-encephalitis group were（0.16±0.08） g/L,（10.45±2.62） mg/L,（0.54±0.38） mg/L,（0.59±0.15） mg/L respectively;and the levels of Ig G and Ig A in the two groups had statistically significant difference（P〈0.05）,however,the levels of Alb and Ig M in the two groups had no statistically significant differences（P〉0.05）.There were 3 cases of the encephalitis group with positive expression of Ig in the OCB,the positive rate was 12%,and the non-encephalitis group was 0%,the positive rate in the two groups had no statistically significant difference（P〉0.05）.Conclusion Increased content of immunoglobulin Ig G and Ig A in CSF can be a biomarkers for diagnosis of children with viral encephalitis,which worthy of clinical application.Oligoclonal zone detection has a certain value for diagnosis of infantile viral encephalitis,need more samples to further research.

关 键 词：脑脊液 免疫球蛋白 寡克隆区带 病毒性脑炎 

分 类 号：R714.04[医药卫生—妇产科学]