萍乡地区AD流行病学调查及CD4^+CD25^+T细胞在AD发病作用的探讨  

Effect of epidemiology and CD4^+CD25^+T cells on the pathogenesis of atopic dermatitis in Pingxiang area

作　　者：吴海虹 林家坤 李运华 李杰模 徐艳萍 

机构地区：[1]江西省萍乡市中医院,江西萍乡337000 [2]江西省萍乡市卫生学校,江西萍乡337000

出　　处：《中国当代医药》2016年第32期139-141,共3页China Modern Medicine

基　　金：江西省卫生计生委科技计划项目(20143276)

摘　　要：目的了解萍乡地区6~13岁儿童特异性皮炎(AD)的发病情况;根据AD患者外周血CD4^+CD25^+T细胞转录因子Foxp3膜分子的表达水平,分析该细胞在AD发病中的作用。方法设置AD患病率调查表,2014年11~12月采用分层整群抽样的方法随机抽取萍乡地区1600名6~13岁的小学生进行调查,选择年龄性别相匹配的健康儿童和AD儿童各50名行流式细胞仪检测,计算出CD4^+CD25^+T细胞转录因子Foxp3膜分子的表达水平。结果萍乡地区6~13岁儿童AD患病率为14.5%,高于全国平均水平的10.2%;萍乡地区城市儿童患病率高于农村儿童(χ~2=4.63,P=0.042),性别差异不大。>10~13岁的儿童患病率低于6~10岁儿童(χ~2=5.36,P=0.039)。AD患者Treg(CD4^+CD25^+)细胞的百分比(0.60±0.18)%明显低于健康儿童的(5.95±1.05)%,差异有统计学意义(t=2.306,P=0.028)。结论 AD可能与环境、食物来源、免疫有关,改善环境、加强食品管理等可降低AD患病率。Objective To understand the incidence of atopic dermatitis in children aged 6-13 years in Pingxiang area;on the basis of the expression level of CD4^＋CD25^＋T cell transcription factor Foxp3 in peripheral blood of patients with AD,the role of this cell in the pathogenesis of AD was analyzed.Methods AD prevalence rate questionnaire was set,from November to December in 2014 1600 pupils of 6-13 years old in Pingxiang area were chosen and investigated by stratified random sampling method,healthy children with age and sex matched,and children with AD were selected and detected by flow cytometry,50 children in each group,the expression level of CD4^＋CD25^＋T transcription factor Foxp3 membrane molecule was calculated.Results Prevalence rate of 6-13 years old children with AD in Pingxiang area was14.5%,higher than 10.2% of national average level;prevalence rate children in Pingxiang area was higher than that of rural children（χ^2=4.63,P=0.042）,there was no gender difference.Prevalence rate of 10-13 years old children was lower than that of 6-10 years old children（χ^2=5.36,P=0.039）.The percentage of Treg（CD4^＋CD25^＋） cells of AD patients was significantly lower than that of healthy children [（0.60＋0.18）% vs（5.95＋1.05）%],and the difference was statistically significant（t=2.306,P=0.028）.Conclusion AD may be related to environment,food sources,immunity,improving the environment and strengthening food management and so on can reduce prevalence rate of AD.

关 键 词：特应性皮炎 儿童 CD4+CD25+T细胞 萍乡地区 

分 类 号：R183.9[医药卫生—流行病学]