Au/TiO_2纳米复合物增强可植入式葡萄糖传感器  

An Au/TiO_2 nanocomposite enhanced biosensor for embedding glucose monitoring

作　　者：刘洋[1] 邹斌[2] 钱鲲[2] 王亚衡 屠一锋[2] 

机构地区：[1]武警总医院药剂科,北京100039 [2]苏州大学材料与化学化工学部,江苏苏州215123 [3]江苏宏锦天药业有限公司,江苏泰州225300

出　　处：《苏州科技学院学报（自然科学版）》2016年第4期48-51,共4页Journal of Suzhou University of Science and Technology （Natural Science Edition）

基　　金：国家自然科学基金资助项目(21375091)

摘　　要：将Au/TiO_2纳米复合材料修饰在针型Pt电极上,用戊二醛作为交联剂将葡萄糖氧化酶固定在此修饰电极上,然后在酶电极上以5%Nafion乙醇溶液涂膜形成高分子膜保护层,制成可植入使用的葡萄糖传感器。采用三电极体系,考察了该传感器的检测能力。实验结果表明,该传感器的检出限为3.0×10^(-6)mol·L^(-1),线性范围为1.0×10^(-5)~1.0×10^(-3)mol·L^(-1)。研究表明,将纳米材料应用于葡萄糖传感器,成功地提高了灵敏度。还考察了影响传感器酶活性的部分因素。该传感器作为一种可植入器件的雏形,有望发展成有实际应用前景的临床检测装备。Based on the modification of Au/TiO2 nanocomposites on a Pt needle electrode ,we fixed the glucose oxidase on it by BSA and glutaraldehyde as the cross-linker. A glucose biosensor was fabricated by coating with 5% Nation to act as a polymer preserving layer. The resulted embeddable glucose biosensor was tested for evaluation of its detection performance. The experimental results show that the linear range of this biosensor is 1.0×10^-5~1.0×10^-3mol·L^-1 with a detection limit of 3.0×10^-6 mol·L^-1. The sensitivity of this needle sensor is promoted by nanomaterials. In addition,the factors affecting the enzyme activity of this biosensor were discussed. As a prototype for implantable usage,the resultant sensor is promising used as a clinical assay device.

关 键 词：血糖 植入式 传感器 葡萄糖氧化酶 Au/TiO2纳米复合物 

分 类 号：O657.1[理学—分析化学]