IgA大鼠肾组织中TGF-β_1的表达与肾损害关联性研究  

The Expression of TGF-β_1 in Renal Tissues and Correlative Study of Renal Damage in Rats With IgA Nephropathy

作　　者：张静[1] 张艳[1] 孙明慧[1] 张蕾[1] 梁新华[1] 

机构地区：[1]新疆医科大学第五附属医院肾病科,乌鲁木齐830000

出　　处：《中国中西医结合肾病杂志》2016年第10期854-857,I0001,共5页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：新疆维吾尔自治区自然科学基金面上项目(No.2015211C169)

摘　　要：目的:检测IgA肾病大鼠血清尿液中转化生长因子-β_1(transforming growth factor beta-1,TGF-β_1)的含量和肾组织中TGF-β_1表达水平,探讨TGF-β_1体内表达与肾损害程度的关联性。方法:将60只SD大鼠随机分为3组,每组20只,以牛血清白蛋白(bovine serum albumin,BSA)灌胃剂量不同分为正常对照组,低剂量组(400 mg/kg),高剂量组(600 mg/kg),各组接受相应的建模干预处理。建模后第8周、12周每组各处死10只大鼠,留取血、尿、肾组织标本检测。酶联免疫吸附法(ELISA)测血清尿液TGF-β_1的含量,免疫组织化学法测TGF-β_1在各组肾组织中的表达。结果:第8、12周时,高剂量组尿液中TGF-β_1的含量和肾组织中MPC-1表达明显高于低剂量组(P<0.05),高剂量组血肌酐、尿素氮及24 h尿蛋白定量明显高于低剂量组,高剂量组间质损害的评分高于低剂量组。三组间血清TGF-β_1的含量差异无统计学意义(P>0.05)。结论:高剂量BSA灌胃可增加IgA肾病大鼠尿液TGF-β_1含量和肾组织中表达,随着尿液TGF-β_1含量和肾组织中TGF-β_1表达水平的增加,肾脏损害程度加重,提示TGF-β_1可作为肾脏损害检测指标。Objective： To investigate the expression of blood and urine transforming growth factor beta- 1,（ TGF- β_1） in rats with Ig A nephropathy models,and to analyse the association between the TGF- β_1expression level and rat kidney different pathological damage degree. Methods： Sixty health SD rats weighing 200 ~ 240 g were randomized into three groups（ 20 pes / group）. Low dose group,the rats received oral BSA 400 mg / kg（ 100 g / L）,the next day lavage for 12 weeks,and subcutaneous injection castor oil0. 3 ml add CCL40. 10 ml,once a week for 12 weeks,8 weeks LPS0. 05 mg caudal vein injection. High dose group,BSA increased to 600 mg / kg（ 100 g / L） in addition to the above treatments. Control group,to physiological saline 4 ml / kg lavage,subcutaneous injection 0. 4 ml physiological saline,frequency with model group. Ten rats were sacrificed respectively in every group at the end of week 8 and 12,the blood,urine,kidney tissue samples were examined. ELISA method（ ELISA） test blood and urine TGF- β_1content,immunohistochemistry SP method to detect TGF- β_1in the kidney tissues,the expression of image analysis to its expression half quantitative analysis. Specification kidney pathological damage is heavier,the expression of TGF- β_1the more significant. Results：8 and 12 weeks,high dose group in urine TGF- β_1content and renal tissues TGF- β_1expression is obviously higher than that of low dose group（ P〈0. 05）,the high dose group of serum creatinine,blood urea nitrogen and 24 h urine protein quantitative is obviously higher than that of low dose group,high dose group of interstitial damage score higher than low dose group. Conclusion： High dose lavage BSA can increase the Ig A nephropathy rats urine TGF- β_1content and renal tissues express,as urine TGF- β_1content and renal tissues TGF- β_1expression level increases,kidney damage degree aggravating,indicating that the TGF- β_1can be used as kidney damage detection index.

关 键 词：IGA 肾病 大鼠 TGF-Β1 

分 类 号：R692[医药卫生—泌尿科学]