β-雌二醇对人脐血管收缩的调节作用  

Effects of β-estradiol on vasoconstriction in human umbilical artery and vein

作　　者：何芸[1] 杨玉贤[1] 竺迪 吴蕾[2] 徐智策[1] 高芹芹 He Fun Fang Yuxian Zhu Di Wu Lei Xu Zhice Gao Qinqin(Institute of Fetology, First Hospital of Soochow University, Suzhou 215006, China Center for Disease Control and Prevention of Suzhou Industrial Zone, Suzhou 215021, China)

机构地区：[1]苏州大学附属第一医院胎儿医学研究所,215000 [2]苏州工业园区疾病防治中心,215021

出　　处：《中华围产医学杂志》2016年第11期855-860,共6页Chinese Journal of Perinatal Medicine

摘　　要：目的 探讨β-雌二醇对人脐血管收缩功能的影响及其相关机制。方法 收集2013年12月至2015年6月在苏州大学附属第一医院出生的足月新生儿的脐带，排除多胎妊娠、妊娠合并症、剖宫产、低出生体重儿，共96例。将脐带标本分离出脐动、静脉，剪成3~5 mm的血管环，分别置于37 ℃、含5 ml Krebs溶液的血管浴槽内，行血管功能试验。根据是否孵育β-雌二醇，作为β-雌二醇组及对照组。研究β-雌二醇对去氧肾上腺素诱导的人脐血管收缩的调节作用，并通过孵育一氧化氮合成酶阻断剂L-单甲基精氨酸（Nω-nitro-L-arginine，L-NMMA）、氟维司群（雌激素受体阻断剂）、吲哚美辛（前列腺素类合成阻断剂），以及去除血管内皮或加入累积浓度佛波醇酯（蛋白激酶C激动剂），分别从血管内皮功能及雌激素受体、蛋白激酶C及前列环素的影响等方面，探讨β-雌二醇对脐动、静脉的调节作用及其机制。对数据进行非线性回归并拟合曲线，采用两样本均数的双因素方差分析。结果（1）脐动、静脉对去氧肾上腺素均有剂量依赖性收缩反应，对照组最大收缩强度分别为氯化钾引起平均收缩强度的（59.17±5.98）%和（43.35±5.02）%，β-雌二醇组则分别为氯化钾的（5.87±1.32）%和（4.52±1.22）%。孵育β-雌二醇使人脐动、静脉对去氧肾上腺素收缩反应曲线右移。（2）在对照组和β-雌二醇组，孵育L-NMMA或者去除内皮，均可增大脐静脉和动脉由去氧肾上腺素所诱导的收缩反应，且这一幅度的差异有统计学意义，但在对照组和β-雌二醇组孵育L-NMMA或者去除内皮后，去氧肾上腺素诱导的收缩反应增加的幅度的差值无统计学差异。（3）不论是否孵育氟维司群，β-雌二醇组对脐静脉及脐动脉对去氧肾上腺素的收缩反应差异无统计学意义（P值均〉0.05），表明抑制并未明显减弱。（4）β-雌Objective To determine the effects of β-estradiol on vasoconstriction in human umbilical artery and vein and its potential mechanisms.Methods Human umbilical cord samples were obtained from 96 term neonates of healthy singleton pregnant women born in the First Hospital of Soochow University between December 2013 and June 2015 （multiple pregnancy, pregnancy complications, cesarean delivery and low birth weight were excluded）. Human umbilical arteries and veins were isolated and suspended in 37 ℃ organ baths containing 5 ml Krebs solution and exposed to β-estradiol followed by phenylephrine （PE） for vasoconstriction test. The subjects were divided into β-estradiol group and control group according to the presence or absence of β-estradiol incubation. To determine the effects and the possible underlying mechanisms of β-estradiol on PE-induced vasoconstriction, human umbilical artery and vein rings were pretreated with Nω-nitro-L-arginine （L-NMMA, nitric oxide synthesis inhibitor）, fulvestrant （ICI182780, estradiol receptor antagonist）, indomethacin （prostaglandin synthesis blocker）, and removal of endothelium, then incubated with β-estradiol for 60 min followed by PE, and the concentration-response curves to PE were recorded. The concentration-response curves to phorbol 12,13-dibutyrate （PDBU, protein kinase C agonist） in Krebs solution in the presence or absence of β-estradiol were also obtained. Nonlinear regression and fitting curve were performed, and the two-sample ANOVA was used for analysis.Results （1） β-estradiol suppressed PE-induced vasoconstriction of human umbilical vein and artery. In human umbilical vein and artery of the control group, the maximum contraction intensity induced by PE was （59.17±5.98）% and （43.35±5.02）% of that induced by potassium chloride, respectively. The maximum contraction induced by PE in β-estradiol group was （5.87±1.32）% and （4.52±1.22）% of that induced by potassium chloride. （2） In both groups, incubation with L-N

关 键 词：脐动脉 脐静脉 血管收缩 雌二醇 

分 类 号：R714.56[医药卫生—妇产科学]