Gab1在胎盘中的表达与妊娠期糖尿病孕妇新生儿出生体重的关系  

Relationship between Gabl expression in placenta and neonatal birth weight in gestational diabetes mellitus

作　　者：王冬雪[1] 张硕[1] 万庆宇[1] 邵文佳[1] 张明明[1] 陈苗苗[1] 宋薇薇[1] Wang Dongxue Zhang Shuo Wan Qingyu Shao Wenjia Zhang Mingming Chen Miaomiao Song Weiwei(Department of Obstetrics and Gynecology, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, China)

机构地区：[1]中国医科大学附属盛京医院妇产科,沈阳110004

出　　处：《中华围产医学杂志》2016年第11期872-877,共6页Chinese Journal of Perinatal Medicine

基　　金：辽宁省自然科学基金（2013021044）

摘　　要：目的 探讨胎盘组织Gab1表达与妊娠期糖尿病（gestational diabetes mellitus，GDM）孕妇新生儿出生体重的关系。方法 2014年10月至2015年5月在中国医科大学附属盛京医院足月剖宫产终止妊娠的单胎妊娠初产妇中，根据新生儿出生体重及是否合并GDM，选择GDM巨大儿组、GDM正常体重组、正常巨大儿组和正常对照组，各30例。剖宫产时取新鲜胎盘组织，采用免疫组织化学方法、Western印迹及实时荧光定量-聚合酶链反应技术检测胎盘组织中Gab1蛋白及mRNA的表达水平。采用方差分析、LSD、Dunnett＇s T3、χ^2检验及Pearson相关分析进行统计学处理。结果 （1）Gab1蛋白表达定位及阳性率比较：Gab1蛋白在人胎盘组织中定位于细胞核。GDM巨大儿组胎盘Gab1蛋白的表达阳性为93%（28/30），高于GDM正常体重组[73%（22/30）]，正常巨大儿组Gab1蛋白的表达阳性率高于正常对照组[73%（22/30）与47%（14/30）]，且GDM巨大儿组Gab1蛋白的表达阳性率高于正常巨大儿组，GDM正常体重组Gab1蛋白的表达阳性率高于正常对照组（χ^2值分别为4.320、4.444、4.320和4.444，P值均〈0.05）。（2）Gab1蛋白和mRNA表达水平：GDM巨大儿组Gab1蛋白表达水平为1.43±0.58，高于GDM正常体重组（1.05±0.67），正常巨大儿组（0.95±0.59）高于正常对照组（0.64±0.38），且GDM巨大儿组高于正常巨大儿组，GDM正常体重组高于正常对照组，差异均有统计学意义（LSD检验，P值均〈0.05）。Gab1 mRNA表达水平比较结果与蛋白表达水平一致。（3）Gab1蛋白在胎盘中的表达水平与新生儿出生体重正相关（r=0.320，P=0.000）。结论 Gab1在胎盘中的表达参与了GDM新生儿出生体重的调节。Objective To evaluate the relationship between placental expression of Gab1 and neonatal birth weight in mothers with gestational diabetes mellitus （GDM）.Methods From the singleton and full-term cesarean delivered women in Shengjing Hospital Affiliated to China Medical University between October 2014 and May 2015, 30 macrosomia babies with maternal GDM were selected as GDM macrosomia group, 30 cases of GDM with normal neonatal birth weight as GDM normal group, 30 cases without GDM but with macrosomia as normal macrosomia group, and 30 cases without GDM and with normal neonatal birth weight as the normal control group. Gab1 protein and mRNA expression in placentas were detected using immunohistochemistry, Western blot and real-time quantitative-polymerase chain reaction. Analysis of variance, LSD, Dunnett＇s T3, Chi-square test and Pearson＇s correlation analysis were used for statistical analysis.Results （1） Gab1 protein location and positive expression rate： Gab1 protein expression in human placenta tissue was located in the nucleus. The positive epression rate of Gab 1 protein in the GDM macrosomia group was higher than in the GDM normal group and normal macrosomia group [93%（28/30）, 73%（22/30） vs 73%（22/30）] and those in the normal macrosomia group and GDM normal group were higher than in the normal control group[47%（14/30）]（χ^2=4.320, 4.320, 4.444 and 4.444, all P〈0.05）. （2） The expression levels of Gab1 protein and mRNA： The expression level of Gab1 protein in the GDM macrosomia group was higher than in the GDM normal group and normal macrosomia group （1.43±0.58 vs 1.05±0.67 and 0.95±0.59）, and that in the normal macrosomia group and GDM normal group were higher than in the normal control group （0.64±0.38） （LSD test, all P〈0.05）. The expression levels of Gab1 mRNA showed the same trend as the expression levels of Gab1 protein in the four groups. （3） Gab1 protein expression level was positively associated with neonatal birth weight （r=0.320, P=0

关 键 词：糖尿病 妊娠 胎盘 衔接蛋白质类 信号转导 出生体重 巨大胎儿 

分 类 号：R714.256[医药卫生—妇产科学]