锁阳中抗氧化活性成分的筛选研究  

Screening antioxidants from Cynomorium songaricum Rupr.

作　　者：王晓飞[1] 李辉[2] 刘铭佩[1] 焦海胜[1] WANG Xiao-fei LI Hui LIU Ming-pei JIAO Hai-sheng(Department of Pharmacy, Lanzhou University Second Hospital, Gansu Lanzhou 730030, China College of Pharmacy, Lanzhou University, Gansu Lanzhou 730000, China)

机构地区：[1]兰州大学第二医院药学部,甘肃兰州730030 [2]兰州大学药学院,甘肃兰州730000

出　　处：《中国医院药学杂志》2016年第21期1852-1855,共4页Chinese Journal of Hospital Pharmacy

基　　金：中央高校基本科研业务费专项资金(编号:lzujbky-2013-46);兰州大学第二医院博士科研基金(编号:Ynbskyjj2-12-1-27)

摘　　要：目的:筛选锁阳中的抗氧化活性成分。方法:采用CUPRAC法确定锁阳抗氧化活性部位。以Cu^(2+)-新亚铜灵试剂为显影试剂的TLC-生物自显影技术结合硅胶柱层析筛选分离活性部位中的抗氧化活性物质,并采用1 H-NMR和13 C-NMR技术确定其的化学结构。结果:确定乙酸乙酯部位为锁阳的抗氧化活性部位,筛分得到的抗氧化活性成分主要有原儿茶酸、儿茶素、没食子酸、儿茶素没食子酸酯。结论:以Cu^(2+)-新亚铜灵试剂为显影试剂的TLC-生物自显影技术能够快速、有效地从锁阳活性部位中筛选出抗氧化活性成分。OBJECTIVE To screen antioxidants fromCynomorium songaricum Rupr..METHODS CUPRAC method was used to screen active fraction of Cynomorium songaricum Rupr..Then TLC-bioautography utilizing Cu2＋-neocuproine reagent and column silicon gel chromatography were combined to screen and separate antioxidants from the active fraction.1H-NMR and 13 C-NMR were used to identify chemical structures of these antioxidants.RESULTS The ethyl acetate fraction was screened as the active fraction.Protocatechuic acid,catechin,gallic acid and epicatechin gallate were screened and separated as antioxidants.CONCLUSION TLC-bioautography utilizing Cu2＋-neocuproine reagent is a fast and effective method to screen antioxidants from the active fraction of Cynomorium songaricum Rupr..

关 键 词：锁阳 TLC-生物自显影技术 Cu2+-新亚铜灵试剂 抗氧化 CUPRAC 

分 类 号：R969[医药卫生—药理学]