双氢睾酮对人毛囊生长影响的初步研究  

Preliminary Study on the Effect of Androgen on Human Follicle Organ Culture in vitro

作　　者：陈贤燕 刘本[1] 李英[2] 陈智睿[1] 梁盼盼[1] 赖维[1] 万苗坚[1] 

机构地区：[1]中山大学附属第三医院皮肤科,广东广州510630 [2]广州市第八人民医院,广东广州510060

出　　处：《皮肤性病诊疗学杂志》2016年第3期156-160,共5页Journal of Diagnosis and Therapy on Dermato-venereology

基　　金：2012年国家自然基金面上项目(编号:81271769)

摘　　要：目的:探讨不同浓度双氢睾酮(DHT)对体外培养人毛囊生长的影响。方法:将分离的人毛囊培养于浓度为10^(-5)mol/L、10^(-6)mol/L、10^(-7)mol/L、10^(-8)mol/L、10^(-9)mol/L的DHT中,设置空白对照组,每日测量毛囊生长长度;运用免疫荧光技术评估各组毛囊毛母质细胞增殖程度。结果:不同浓度DHT对体外培养的毛囊生长产生不同影响:DHT培养浓度为10^(-5)mol/L、10^(-6)mol/L时抑制毛囊生长(正常对照组与DHT 10^(-5)mol/L、10^(-6)mol/L实验组毛囊每日平均生长长度相比,P值均<0.05),毛母质细胞增殖活性减弱,毛囊较早进入退行期;DHT培养浓度为10^(-7)mol/L时促进毛囊生长(正常对照组与DHT10^(-7)mol/L实验组毛囊每日平均生长长度相比,P<0.05),毛母质细胞增殖活性强,毛囊生长期(anagen)延长;DHT培养浓度为10^(-8)mol/L、10^(-9)mol/L时对毛囊生长的作用与对照组相比无明显差异(正常对照组与DHT 10^(-8)mol/L、10^(-9)mol/L实验组毛囊每日平均生长长度相比,P值均>0.05)。结论:不同浓度DHT对体外培养毛囊生长的影响不同。Objective：To examine the effect of different concentrations of androgen （ di-hydrotestosterone;DHT） on human follicle in vitro .Methods：Follicles were isolated and cultured with DHT of different concentrations from 10 -5 mol/L to 10 -9 mol/L.Then the growth rate of the follicles with or without DHT was observed , ki67 was used to assess the proliferation activity of hair matrix cells from each cultured hair follicle .Results：DHT at concentrations of 10 -5 mol/L and 10 -6 mol/L inhibited the growth of hair follicles compared with the control group （ P〈0.05） , the hair matrix cell proliferation activity was weakened , and follicle entry into catagen phase was earlier than that of the control group .However, DHT at a concentration of 10 -7 mol/L promoted the growth of hair follicles compared to the control group （ P〈0.05 ） ,the hair matrix cell prolifera-tion activity was strengthened , and follicle entry into catagen phase was later than that of the con-trol group.The effect of DHT on hair follicles at concentrations of 10 -8 mol/L and 10 -9 mol/L showed no significant difference compared to the control group （P〉0.05）.Conclusion：Different concentrations of DHT had different effects on in vitro hair follicles .

关 键 词：毛囊培养 雄激素 双氢睾酮 浓度 

分 类 号：R758.71[医药卫生—皮肤病学与性病学]