盐酸法舒地尔对脂多糖诱导人冠状内皮细胞缝隙连接蛋白43表达的影响  

THE REGULATION OF HYDROXYFASUDIL ON LPS-MEDIATED CONNEXIN43 EXPRESSION IN HUMAN CORONARY ENDOTHELIAL CELLS

作　　者：王淳[1] 巫相宏[1] 黄文[2] 马国添[1] 闭奇[1] 李世云[1] Wang Chun Wu Xianghong Huang Wen Ma Guotian Bi Qi Li Shiyun(Department of Cardiology,The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China)

机构地区：[1]广西医科大学第一附属医院心血管内科,南宁530021 [2]广西医科大学第一附属医院神经内科,南宁530021

出　　处：《广西医科大学学报》2016年第3期382-385,共4页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.81360057)

摘　　要：目的:探讨盐酸法舒地尔(HF)对脂多糖(LPS)诱导人冠脉内皮细胞(HCAEC)中缝隙连接蛋白43(Cx43)表达的影响。方法:用不同浓度的LPS(0.01 mg/L、0.1 mg/L、1 mg/L、10 mg/L)及不同浓度的HF(10μmol/L、30μmol/L、50μmol/L、80μmol/L)分别刺激HCAEC,采用MTT法检测细胞活力。将HCAEC随机分为空白对照组、LPS组、HF组和LPS+HF组,分别采用Western blotting和免疫组化法检测HCAEC Cx43蛋白的表达。结果:LPS浓度≥1mg/L时,HCAEC活力显著降低(P<0.05);HF浓度≥50μmol/L时,HCAEC活力显著降低(P<0.05)。与空白对照组相比,LPS组HCAEC Cx43蛋白表达上调(P<0.05),HF组HCAEC Cx43蛋白表达下调(P<0.05);LPS预处理细胞1h后与HF共培养,与LPS组相比,LPS+HF组HCAEC中Cx43的表达下调(P<0.05)。结论:HF能抑制LPS诱导的HCAEC中Cx43蛋白表达的增加,保护内皮间缝隙连接,从而发挥抗动脉粥样硬化的作用。Objective：To evaluate the role of hydroxyfasudil（HF）in lipopolysaccharide（LPS）-mediated connexin 43（Cx43）expression in human coronary endothelial cells（HCAEC）.Methods：HCAEC were exposed to five different concentrations of LPS（0mg/L,0.01mg/L,0.1mg/L,1mg/L and 10mg/L）and HF（0μmol/L,10μmol/L,30μmol/L,50μmol/L and 80μmol/L）respectively,followed by MTT assay to test cell viability.HCAEC were randomly divided into the control group,LPS group,HF group and LPS＋HF group.Cx43 expression was detected by western blotting and immunohistochemistry in HCAEC after24 htreatment.Results：The viability of HCAEC was significantly inhibited when the concentration of LPS was increased to 1mg/L（P〈0.05）or the concentration of HF was increased to 50μmol/L（P〈0.05）.Compared with the control group,the expression of Cx43 was up-regulated in the LPS group（P〈0.05）,while it was down-regulated in the HF group（P〈0.05）.Furthermore,the expression of Cx43 was significantly down-regulated when co-treatment with LPS and HF（P〈0.05）.Conclusion：HF exerts an effect of anti-atherosclerosis by suppressing LPS-induced Cx43 expression in HCAEC and enhancing formation of endothelial gap junctions.

关 键 词：盐酸法舒地尔 缝隙连接蛋白43 脂多糖 人冠脉内皮细胞 

分 类 号：R541.4[医药卫生—心血管疾病]