氨烷基磷酸钠及其改性的羟基磷灰石对MG63细胞功能的影响  

The effects ofaminoalkyl phosphates and their modified hydroxyapatites on MG63 cell functions

作　　者：刘帅[1] 孙富华[1] 江依柔 谢兴益[1] 

机构地区：[1]四川大学高分子科学与工程学院,成都610065

出　　处：《功能材料》2016年第6期108-112,共5页Journal of Functional Materials

基　　金：国家自然科学基金资助项目(50973069)

摘　　要：氨烷基磷酸钠（AAP-n-Na,n=2-6,为氨烷基的碳原子数）可用于制备羟基磷灰石（HA）胶体,是新型的HA表面改性剂,我们的前期研究已证实其无细胞毒性。进一步研究APP-n-Na及其改性HA（Cn-HA）对成骨样细胞MG63功能的影响。研究结果表明,在0.1%浓度下,n=3,4,5的APP-n-Na对MG63细胞的碱性磷酸酶（ALP）和骨钙素（OCP）的表达无不良影响,和阴性对照（空白培养基）的表达水平一致;所有APP-n-Na对矿化结节的形成无不良影响。Cn-HA的浸提液（0.1g/mL,以培养基为浸提介质）对ALP的表达和生物矿化均无不良影响,和对照样（未改性HA和空白培养基）相比无显著差异。研究预示APP-n-Na及其改性的HA用于骨修复材料是安全的。Omega-aminoalkyl sodium hydrogen phosphates （AAP-n-Na,n=2-6,representing the carbon num-ber in the alkyl group）can be used to prepare hydroxyapatite （HA）hydrocolloids,which are new surface modi-fiers for HA and have no cytotoxicity as confirmed previously.In this study,the effects of AAP-n-Na and their modified HAs （Cn-HA,n=2-6）on the functions of human osteoblast-like MG 63 cells were further investiga-ted.The results showed that the alkaline phosphatase （ALP）and osteocalcin （OCN）expressions in cells treated with 0.1% （w/v）APP-n-Na （n=3,4 or 5）in the culture media were comparable to the expressions in the blank medium （DMEM supplemented with 10% fetal bovine serum）as a negative control;and all AAP-n-Na’s （n=2-6）did not inhibit the calcium nodule formation.The extracts from corresponding Cn-HAs （prepared at 0.1 g/mL in the cell culture medium）did not significantly alter the ALP expression and calcium nodule forma-tion in MG 63 cells as well,in comparison with the bare HA extract and the blank medium.These results imply that AAP-n-Na’s and their modified HAs are safe when used in bone grafting materials.

关 键 词：氨烷基磷酸 羟基磷灰石 成骨细胞 碱性磷酸酶 骨钙素 钙化结节 

分 类 号：R318[医药卫生—生物医学工程] TQ132.3[医药卫生—基础医学]