大蒜微型鳞茎诱导培养基的筛选初报  

A Preliminary Report on Screen the Induction Medium of Garlic Micro Bulb

作　　者：王云[1] 蒲建刚[1] 王德贤[1] 常德昌[2] 王琰[1] 缑建民[1] 葛亮[1] 

机构地区：[1]甘肃省天水市农业科学研究所,甘肃天水741001 [2]甘肃省天水市果树研究所,甘肃天水741002

出　　处：《甘肃农业科技》2016年第6期21-23,共3页Gansu Agricultural Science and Technology

基　　金：甘肃省科技支撑计划项目"陇东南大蒜脱毒快繁技术研究及示范"(1204NKCE105)部分内容

摘　　要：以吉木萨尔白蒜为试验材料,利用大蒜鳞茎盘为外植体,在B5＋6-BA0.5 mg/L＋NAA0.5 mg/L＋蔗糖30 g/L＋琼脂4.5 g/L培养基进行初始培养获得绿芽,在此基础上开展试管内诱导微鳞茎培养基配方研究。结果表明,1/2 MS＋IBA 1.5 mg/L＋NAA 0.1 gm/L＋蔗糖50 g/L＋琼脂4.5 g/L为组培苗试管内诱导鳞茎较好的培养基配方,在该培养基上大蒜绿芽第45天开始形成鳞茎,第90天鳞茎直径为0.3~0.5 cm。The Jimsar white garlic as experimental materials, using garlic bulb as explants, Initial culture is carried out to obtain green bud in BS＋6-BA 0.5 mg/L ＋NAA0.5 mg/L ＋ sucrose 30 g/L ＋ agar 4.5 g/L medium, on the basis of this study, the formula of the culture medium for inducing the micro bulb in vitro is developed. The result shows that 1/2 MS＋IBA 1.5 mg/L＋NAA 0.1 gm/L 1/2 MS ＋ IBA 1.5 mg/L ＋ sugar ＋ 50 g/L ＋ agar 4.5 g/L is a better culture medium formula for inducing bulb in tissue culture plantlet, the garlic green shoots began forming a bulb the in 45th day, the bulb diameter up to 0.3-0.5 cm in 90th day under the culture medium.

关 键 词：组织培养 鳞茎盘 诱导 培养基 大蒜 

分 类 号：S633.4[农业科学—蔬菜学]