悬浮芯片法调查广州腹泻病原体分布及临床特征分析  

Distribution of enteropathogens detected by the Luminex x TAG Gastrointestinal Pathogen Panel and clinical characteristics of diarrhea patients in Guangzhou

作　　者：林丽娟[1] 彭雅冰 罗欣[2] 陈满君 邓间开[1] 余楠[1] 

机构地区：[1]南方医科大学珠江医院检验医学部,广东省突发传染病病原微生物重点实验室,广州市510282 [2]广东省妇幼保健院检验科,广州市511400

出　　处：《实用医学杂志》2016年第22期3724-3727,共4页The Journal of Practical Medicine

基　　金：传染病防治重大专项课题(编号:2012ZX10004213-006)

摘　　要：目的:了解引起腹泻病原体种类随季节、年龄的分布,病原体种类与症状、实验检查结果的关系。方法:收集2014-2015年腹泻者粪便326例,使用液相悬浮芯片法检测15种病原体。结果:诺如病毒GⅠ/GⅡ型(15.6%,51/326)、A组轮状病毒(14.4%,47/326)和弯曲菌(12.6%,41/326)检出最多。寄生虫20例(6.13%),合并感染51例(15.6%)。秋冬季以A组轮状病毒(13.2%,43/326)、诺如病毒GⅡ型(9.8%,32/326)、弯曲菌(9.5%,31/326)为主。儿童(<14岁)A组轮状病毒(11.9%,39/326)常见,病原检出率较成人高。病毒与细菌感染引起隐血(P<0.001)、白细胞升高(P<0.001)、呕吐(P<0.01)比率有差异。结论:研究提供了腹泻病原体种类随季节、年龄分布的情况;细菌性与病毒性腹泻临床表现和实验检查有差异。Objective To present the distribution of enteropathogens and the clinical characteristics of diarrhea patients in Guangzhou. Methods Stool samples were collected from diarrhea patients going to Zhujiang Hospital during September 2014 to September 2015. Nucleic acid was extracted and 15 enteropathogens, including bacteria, viruses and parasites, were detected subsequently by Luminex xTAG Gastrointestinal Pathogen Panel （GPP）, an assay based on the liquid phase suspension chip technology. Demographic data, clinical notes and laboratory findings for all patients were reviewed. Results It was found that the norovirus GI/GII （15.0%, 51/326）, rotavirus A （14.4%, 47/326） and campylobacter （12.6%, 41/326） were detected mostly. Parasites were detected in 20 specimens （6.13%, 20/326）. Co-infections were observed in 50 cases （15.3%, 50/326）, including 44 double, 6 triple and 1 quadruple infections. The pathogens were detected mainly in autumn and winter, with rotavirus A （43/326）, norovirus GII （32/326） and campylobacter （31/326） the top three commonly detected. Pediatric patients （〈 14 years） were more likely detected than that in adults, with rotavirus the most common pathogen detected in children （39/326）. When comparing infections caused by bacteria and virus, fecal occult blood （P 〈 0.001）, white blood cells （P 〈 0.001） in feces were more common in bacterial diarrhea, while vomiting （P 〈 0.01）, instead of fever （P = 0.334）, was more frequently observed in viraldiarrhea. Conclusion The distributions of the enteropathogens with varied season and age of diarrhea patients were presented in the study. Significant difference of occult blood, white blood cells in feces and vomiting was observed between viral and bacterial infections.

关 键 词：腹泻 病原分布 液相悬浮芯片法 临床特征 

分 类 号：R446.5[医药卫生—诊断学]