吡格列酮对MZT期小鼠胚胎发育阻滞的影响  

Effect of Pioglitazone on Development Block of Mouse Embryos during Maternal to Zygotic Transition

作　　者：徐礼杰[1] 李钟淑[1] 方南洙[1] XU Li-jie LI Zhong-shu FANG Nan-zhu(Department of Animal Science, Agricultural College of Yanbian University, Yanji 133002, China)

机构地区：[1]延边大学农学院动物科学系,延边133002

出　　处：《畜牧兽医学报》2016年第11期2240-2247,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(31360546)

摘　　要：本研究旨在了解活性氧(Reactive oxygen species,ROS)、发育阻滞与抗氧化物吡格列酮(Pioglitazone,PIO)之间的关系。试验分3组:对照组、H_2O_2处理组和H_2O_2+PIO处理组。通过H_2O_2建立小鼠胚胎氧化损伤模型,添加抗氧化物质PIO对胚胎进行氧化修复,DCHFDA测定胚胎内部ROS水平,半定量PCR测定母型-合子型胚胎内部相关母性效应基因和合子型基因的表达。结果表明:(1)1-细胞和2-细胞胚胎H_2O_2处理组的ROS水平明显比对照组和H_2O_2+PIO处理组的高(P<0.05);(2)H_2O_2处理组的2-细胞向4-细胞过渡率明显比对照组和H_2O_2+PIO处理组低(P<0.05),但各处理组之间1-细胞向2-细胞过渡率差异不显著(P>0.05);(3)H_2O_2处理组的母性效应基因Hsf1、Zfp3612、Zp3和合子型基因Eif1-α、Zscan4d和Muerv-L的表达明显比对照组和H_2O_2+PIO处理组低(P<0.05)。综上表明,氧化应激通过下调2-细胞胚胎母型效应基因和合子型基因表达,导致小鼠胚胎2-细胞发育阻滞,抗氧化物质吡格列酮可促进胚胎发育,有效克服胚胎体外2-细胞发育阻滞现象。The aim of this study was to investigate the relationship of ROS,developmental block and pioglitazone as an antioxidant.The experiment was divided into 3groups：Control group,H2O2 group and H2O2＋PIO group.The oxidative damage model of mouse embryos induced by H2O2 was established and treated with or without pioglitazone.The ROS level after treatment with pioglitazone was measured by DCHF-DA and the expression of maternal effect genes and zygotic genes were analyzed by Semi-quantitative reverse transcription PCR.The results showed that：（1）the ROS level of H2O2 group was significantly higher than control group and H2O2＋PIO group in both1-cell and 2-cell embryos（P〈0.05）;（2）the rate of 2-cell to 4-cell transition in H2O2 group was significantly lower than control group and H2O2＋PIO group（P〈0.05）,while the difference of 1-cell to 2-cell transition rate among the groups was not significant（P〉0.05）;（3）the expression of maternal effect genes（Hsf1,Zfp3612,Zp3）and zygotic genes（Eif1-α,Muerv-L,Zscan4d）in H2O2 group was lower than control group and H2O2＋PIO group（P〈0.05）.The results provided a clear molecular aspect regarding the mechanism by which pioglitazone promoted embryos development and overcome 2-cell block.

关 键 词：吡格列酮 氧化应激 发育阻滞 母性效应基因 合子型基因 

分 类 号：Q813.7[生物学—生物工程]