副猪嗜血杆菌potD基因缺失株的构建及其部分生物学特性  

Construction and Characterization of a Haemophilus parasuis potD Mutant Strain

作　　者：谨瑾 张禄滑 文心田[1] 李英[1] 代科[1] 周鹏[1,2] 赵玉佳[1] 曹三杰[1] 黄小波[1] 伍锐[1] 赵勤[1] 文翼平[1] JIN Jin ZHANG Lu-hua WEN Xin-tian LI Ying DAI Ke ZHOU Peng ZHAO Yu-jia CAO San-jie HUANG Xiao-bo WU Rui ZHAO Qin WEN Yi-ping(Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China Panzhihua Bureau of Agriculture and Animal Husbandry, Panzhihua 617000, China)

机构地区：[1]四川农业大学动物医学院猪病研究中心,成都611130 [2]四川省攀枝花市农牧局,攀枝花617000

出　　处：《畜牧兽医学报》2016年第11期2274-2279,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：公益性行业(农业)科研专项(201303034)

摘　　要：potD基因编码的蛋白质作为转运体蛋白,负责多胺的结合和转运,为细胞的正常生长提供必需因子,已在多种病原菌中鉴定为毒力相关因子,但在副猪嗜血杆菌中的作用还不清楚。利用自然转化法构建potD缺失株SC1401ΔpotD::kan,再比较亲本株和缺失株的生长特性、自凝集活性、生物被膜形成能力、抗血清杀菌能力以及对小鼠的致病力。结果显示potD基因的缺失不影响副猪嗜血杆菌的生长、自凝集活性和生物被膜形成能力,但导致该菌的抗血清杀菌能力和对小鼠的致病力显著降低。以上结果表明potD基因可能与副猪嗜血杆菌的毒力相关。As a transporter,protein encoded by potDis involved in the binding and transportation of polyamine,which is necessary for the growth of cells.In some pathogens,the protein is identified as a virulence-associated factor,but of which the function is still unclear in Haemophilus parasuis.ApotD mutant strain SC1401ΔpotD：：kan was constructed by the natural transformation method.The growth curve,the ability of autoagglutination and biofilm formation,serum resistance ability and the virulence to mice of the parental strain SC1401 and potD mutant strain were measured.No significant difference was observed between the parental and mutant strains in the growth curve and the ability of autoagglutination and biofilm formation.However the potD mutant strain showed an obvious decrease in serum resistance ability and the virulence to mice.The findings above suggested that potDgene may be associated with virulence in H.parasuis.

关 键 词：副猪嗜血杆菌 potD 毒力 自然转化 缺失株 

分 类 号：S852.613[农业科学—基础兽医学]