微小RNA-30c、196a2基因多态性与宫颈癌关系的病例-对照研究  

Case-Control Study on Relationship Between Polymorphisms of MicroRNA-30c and MicroRNA-196a2 and Cervical Cancer

作　　者：王春桃[1] 徐敏 张艳秋[3] 申娴[3] 隋静[3] 梁戈玉[3] 

机构地区：[1]盐城卫生职业技术学院,江苏盐城224001 [2]盐城市第一人民医院,江苏盐城224005 [3]环境医学工程教育部重点实验室/东南大学公共卫生学院,江苏南京210009

出　　处：《环境与职业医学》2016年第11期1043-1048,共6页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金(编号:81472939);江苏省高校哲学社会科学研究项目(编号:2016SJB880119);江苏省六大人才高峰项目(编号:2013-WSW-053);江苏省333工程;盐城市医学科技发展计划项目(编号:YK2013060)

摘　　要：[目的]探讨微小RNA（mi R）-30c和mi R-196a2基因多态性与宫颈癌的相关性。[方法]于2012年9月—2014年9月以病例-对照研究的方法收集来自盐城市第一人民医院的104例宫颈癌患者及186例对照,采用多重聚合酶链反应（PCR）和多重连接酶检测反应（LDR）技术对病例组和对照组的mi R-30c rs928508和mi R-196a2 rs11614913基因多态性进行分析,采用卡方检验分析各基因型与宫颈癌发生之间的相关性。[结果]mi R-30c rs928508 GG、AG和AA基因型在病例组和对照组中的分别为16.3%、51.9%、31.8%和29.0%、47.3%、23.7%,差异具有统计学意义（P〈0.05）。与GG基因型比较,携带AG和AA基因型的个体患宫颈癌的风险明显升高（P〈0.05）,其中携带AA基因型个体危险度增加至2.38（95%CI：1.17~4.83）。未发现mi R-196a2 rs11614913多态性与宫颈癌发生具有相关性（P〉0.05）。[结论]mi R-30c rs928508基因多态性与宫颈癌发生相关。[ Objective ] To assess the relationship between polymorphisms of MicroRNA（miR）-30c and miR-196a2 and cervical cancer. [ Methods ] A case-control design was carried out in this study, which included 104 patients with cervical cancer and 186 controls from Yancheng City No.1 People＇s Hospital from September 2012 to September 2014. The gene polymorphisms of miR-30c rs928508 and miR-196a2 rs11614913 in patients and controls were analyzed by multiplex polymerase chain reaction and multiple ligase detection reaction. The association between identified genotypes and cervical cancer was analyzed by chi-square test. [ Results ] The genotype of miR-30c rs928508 were 16.3% for GG, 51.9% for AG, and 31.8% forAA in the cases and 29.0% for GG, 47.3% for AG, and 23.7% for AA in the controls, and there were statistical differences between the cases and the controls （P〈 0.05）. Compared with GG genotype, AG or AA genotype was significantly associated with an increased risk of cervical cancer （P〈 0.05）, and the OR was 2.38 for individuals with AA genotype versus those with GG （95%CI： 1.17-4.83）. No significant association was found between gene polymorphism of miR-196a2 rs11614913 and cervical cancer （P 〉 0.05）. [ Conclusion ] The miR-30c rs928508 polymorphism may be correlated with cervical cancer.

关 键 词：miR-30c miR-196a2 基因多态性 宫颈癌 病例-对照研究 

分 类 号：R181.33[医药卫生—流行病学]