康脑液对脑缺血再灌注大鼠神经再生相关因子的影响  

Effect of Kangnaoye on Nerve Regeneration Related Factor in Rats of Cerebral Ischemia-Reperfusion Injury

作　　者：孙炎[1] 宋爱霞[2] 薛茜[2] 邹玉安[2] SUN Yan SONG Ai-xia XUE Qian ZOU Yu-an(Hebei North University, Zhangjiakou, 075000, China Department of Neurology, the First Affiliated Hospital of Hebei North University, Zhangjiakou, 075000, China)

机构地区：[1]河北北方学院 [2]河北北方学院附属第一医院神经内科

出　　处：《神经药理学报》2015年第5期7-14,共8页Acta Neuropharmacologica

基　　金：河北省卫生厅课题基金资助项目(No.20090591)

摘　　要：目的:观察康脑液对脑缺血再灌注大鼠神经再生相关因子的影响。方法:150只健康雄性SD大鼠随机分为高剂量康脑液组(Kangnaoye high dose group,KNYH)、中剂量康脑液组(Kangnaoye middle dose group,KNYM)、低剂量康脑液组(Kangnaoye low dose group,KNYL)(分别为24、12、6 g·kg^(-1)·d^(-1)),假手术组(sham group)及模型组(model group)。用线栓法制备大脑中动脉闭塞模型,于缺血后2h再灌注,24 h后TTC染色测各组脑梗死体积;分别在1、3、7、14 d时处死大鼠采集标本,用免疫组织化学法观察脑缺血区生长相关蛋白(growth associated protein-43,GAP-43)和勿动蛋白(neurite outgrowth inhibitor-A,NOGO-A)的表达情况;同时分别于再灌注后2 h、24 h、3 d、7 d、14 d评价动物神经功能。结果:康脑液各剂量组大鼠神经功能评分明显低于模型组,差异有统计学意义(P<0.05);与模型组相比,康脑液各剂量组大鼠的梗死体积明显缩小,差异有统计学意义(P<0.01);康脑液高、中剂量组梗死灶周边区脑组织GAP-43表达较模型组显著升高,NOGO-A表达较模型组显著降低,差异有统计学意义(P<0.05或P<0.01)。结论:康脑液可促进脑缺血再灌注大鼠的神经再生,从而改善脑梗死大鼠运动功能。Objective：To observe the effect of Kangnaoye on nerve regeneration related factor in rats of cerebral ischemia-reperfusion injury.Methods：150 healthy adult male Sprague Dawley rats were randomly divided into 5 groups;Kangnaoye high,middle and low dose group（24、12、6g·kg^（-1）·d^（-1）,sham operated group and model group.Longa method was used to establish the middle cerebral artery occlusion（MACO） model.TTC staining method was used to observe infarction volume change in rats after 24 h.Collecting specimens in 1 d、3 d、7 d and 14 d,immunohistochemical method was used to observe the change of the growth associated protein-43（GAP-43） after cerebral ischemic and the expression of neurite outgrowth inhibitor-A（NOGO-A） protein.The nervous function deficit scores were evaluated at 2 h、1 d、3 d、7 d、14 d after reperfusion.Results：Compared with model group,the neurological functions were improved significantly in kangnaoye groups（P0.05）.Kangnaoye high,middle and low dose groups were significantly decreased than model group in cerebral infarction volume ratio（P0.01）.The expression levels of GAP-43 were also significantly higher in kangnaoye high and middle dosages groups than model group,at the same time the expression of NOGO-A were lower,which has significant differences（P0.05 or P0.01）.Conclusion：Kangnaoye can promote neurotization and improve the motor skill recovery after cerebral infarction.

关 键 词：康脑液 脑缺血再灌注 神经再生 

分 类 号：R965[医药卫生—药理学] R743[医药卫生—药学]