电针迎香穴对嗅觉障碍大鼠嗅粘膜Ki-67、IGF-1R及嗅球IGF-1表达的影响  

Effects of electroacupuncture of Yingxiang on expression of Ki-67 and IGF-1R of olfactory mucosa and IGF-1 of olfactory bulb in rats with olfactory dysfunction

作　　者：王强[1] 刘智斌[1] 牛文民[1] 杨晓航[1] 王渊[1] 朱先伟[1] 刘思洋[2] 王卫刚[3] Wang Qiang Liu Zhibin Niu Wenmin(Shaanxi University of Chinese Medicine （Xianyang 712046)

机构地区：[1]陕西中医药大学,咸阳712046 [2]西安医学院,西安710021 [3]陕西中医药大学附属医院,咸阳712000

出　　处：《陕西中医》2016年第11期1547-1550,共4页Shaanxi Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81273859);陕西省教育厅专项科学研究项目(11JK0679)

摘　　要：目的:观察电针迎香穴干预嗅觉障碍大鼠嗅粘膜Ki-67、IGF-1R及嗅球IGF-1表达的影响。方法:取SD雄性大鼠40只,随机分为空白组,嗅觉障碍组,嗅觉障碍+电针迎香穴组,嗅觉障碍+眶下神经切断+电针迎香穴组,每组10只。除空白组外,其余各组均用灌注Triton X-100的方法建立嗅觉障碍,造模成功后进行电针干预10d,用免疫组织化学法及Western blot法测定组织中Ki-67、IGF-1和IGF-1R的表达水平。结果:嗅觉障碍模型中嗅黏膜Ki-67、IGF-1R和嗅球IGF-1表达减少(P<0.05),电针迎香穴可以显著增加嗅黏膜Ki-67、IGF-1R和嗅球IGF-1的表达水平(P<0.05),而电针迎香穴+眶下神经切断组则无显著干预效应。结论 :电针迎香穴干预可以改善TritonX-100诱导的大鼠嗅觉功能障碍,其机制可能与提高嗅觉神经相关细胞因子Ki-67和IGF-1、IGF-1R在嗅黏膜及嗅球的表达有关,且迎香穴的干预效应与三叉神经通路的完整性密切相关。Objective：To observe the effect based on trigeminal nerve pathway of electroacupuncture of＂Yingxiang＂（LI20）on Ki-67 and IGF-1Rof olfactory mucosa and IGF-1of olfactory bulb of olfactory dysfunction（OD）rats.Methods：40SD mouses were divided into four groups：control group、model group、OD ＋ LI20 group and OD ＋ONT ＋ LI20 group,OD models were established by perfused Triton X-100.10 days were taken as one treatment course.We monitored behavioral detection of rats and observed the expression level Ki-67 and IGF-1Rof olfactory bulb or olfactory mucosa by immuno histochemistry.Results：According to olfactory dysfunction model,expression of Ki-67 and IGF-1R of olfactory mucosa and IGF-1of olfactory bulb were significantly decreased by electroacupuncture group（P〈0.05）,meanwhile,there was no significant difference for electroacupuncture group on ONT of OD rats.Conclusion：Electroacupuncture on LI20 had a favorable effect on Triton X-100-induced olfactory disorders,its mechanism might based on increasing the expression of Olfactory nerve related cytokines Ki-67 and IGF-1Rof olfactory mucosa and IGF-1of olfactory bulb,and the intervention effect might be relied on the integrity of trigeminal nerve pathway.

关 键 词：嗅觉障碍/针灸疗法 穴 迎香 电针 大鼠 动物实验 

分 类 号：R765[医药卫生—耳鼻咽喉科]