云南汉族γ-干扰素受体基因多态性与动脉粥样硬化斑块稳定性的相关性  

Correlation between IFNGR gene polymorphism and atherosclerotic plaque stability in Yunnan Han nationality

作　　者：杨天睿[1] 撒亚莲[2] 

机构地区：[1]云南省第一人民医院内干科,昆明650032 [2]云南省第一人民医院基研室,昆明650032

出　　处：《重庆医学》2016年第33期4643-4645,4649,共4页Chongqing medicine

摘　　要：目的探讨云南汉族γ-干扰素受体(IFNGR)的两个氨基酸位点Val14Met和GIn64Arg多态性与动脉粥样硬化(AS)斑块稳定性的相关性。方法收集该院2014年3月至2015年3月收治的AS斑块不稳定的患者作为观察组,而同期入院的AS斑块稳定/无斑块的患者为对照组。采集患者外周静脉血,提取基因组DNA,通过聚合酶链反应(PCR)产物直接测序法检测IFNGR1Vall4Met和IFNGR2Gln64Arg位点的基因型,采用DNAStar、GeneTool软件分析测序结果,用流式细胞术检测患者血浆细胞因子[γ-干扰素(IFN-γ)]的水平。结果 204例当地汉族患者列入研究,其中观察组109例,年龄(76.89±12.08)岁;对照组95例,年龄(65.99±16.32)岁。IFNGR1Vall4Met位点在观察组和对照组中均未发现多态性改变。IFNGR2Gln64Arg在观察组AA基因型频率为51.95%(40/77),AG基因型频率为53.06%(52/98),GG基因型频率为58.62%(17/29);对照组AA基因型频率为48.05%(37/77),AG基因型频率为46.94%(46/98),GG基因型频率为41.38%(12/29),卡方检验P=0.824,IFNGR2基因型AA、AG和GG与AS斑块稳定性没有关系;观察组IFNGR2A等位基因频率为52.38%(132/252),G等位基因频率为55.13%(86/156),对照组A等位基因频率为47.62%(120/252),G等位基因频率为44.87%(70/156),卡方检验校正P=0.661,IFNGR2等位基因A和G与AS斑块稳定性没有关系。经Hardy-Weinberg遗传平衡检验,该样本人群该基因频率符合遗传平衡法则。观察组血浆中IFN-γ水平为(4.60±1.91)ng/mL,对照组为(4.88±2.10)ng/mL,差异无统计学意义(P=0.318);血浆IFN-γ水平与AS斑块稳定性无相关性(P=0.308)。结论 IFNGR基因多态性不能作为AS斑块稳定性的预警指标。Objective to explore the correlation between interferon-γreceptor（IFNGR）2amino acid sites Val14 Met and GIn64 Arg polymorphism and atherosclerosis plaque stability in Yunnan Han nationality.Methods The patients with unstable atherosclerotic plaque in our hospital from March 2014 to March 2015 were collected as the observation group and contemporaneous patients with stable atherosclerotic plaque/non- plaque as the control group.The peripheral venous blood was collected for extracting genomic DNA.IFNGR Val14 Met and GIn64 Arg loci genotype was detected by the PCR product direct sequencing method.The sequencing results were analyzed by adopting the DNAStar and GeneTool software.The levels of plasma cytokines（IFN-γ）was detected by the flow cytometry.Results Two hundreds and four native Han patients were included in this study,including the observation group,109 cases,age（76.89±12.08）years old;the control group,95 cases,age（65.99±16.32）years old.The polymorphism change of IFNGR1Vall4 Met loci was not found in the two groups.In the observation group,the frequency of IFNGR2Gln64 Arg genotype AA was 51.95%（40/77）,which of AG was 53.06%（52/98）and which of GG was 58.62%（17/29）;in the control group,the frequencies were 48.05%（37/77）,46.94%（46/98）and 41.38%（12/29）,chi-square test,P=0.824.The IFNGR2Gln64 Arg genotypes AA,AG and GG had no relation with atherosclerotic plaque stability.The A and G allele frequencies in the observation group were 52.38%（132/252）and 55.13%（86/156）respectively,which of the control group were 47.62%（120/252）and 44.87%（70/156）,chi-square test′s P=0.661.The IFNGR2Gln64 Arg A/G allele had no relation with atherosclerotic plaque stability.By Hardy-Weinberg genetic balance test,the gene frequency in this sample population was in accordance with the genetic equilibrium law.The plasma IFN-γlevel in the observation group was（4.60±1.91）ng/mL,which in the control group was（4.88±2.10）ng/mL,the difference was not statistically significant

关 键 词：动脉粥样硬化 γ-干扰素受体 基因 多态性 

分 类 号：R543.5[医药卫生—心血管疾病]