从内质网应激途径探讨淫羊藿总黄酮对衰老大鼠心肌细胞的保护作用  

Protective Effects of Total Flavonoids of Epimedium on Myocardial Cells of Aging Rats Through Endoplasmic Reticulum Stress Pathway

作　　者：王佼[1] 袁丁[2] 宋亚男[1] 周志勇[1] 王婷[1,3] 

机构地区：[1]三峡大学医学院,湖北宜昌4430022 [2]三峡大学附属仁和医院,湖北宜昌4430022 [3]三峡大学缺血性心脑疾病转化医学重点实验室,湖北宜昌443002

出　　处：《中药新药与临床药理》2016年第6期745-750,共6页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金(81273895);三峡大学重点实验室开放基金(2016KXN05)

摘　　要：目的研究淫羊藿总黄酮(TFE)对自然衰老大鼠心肌细胞的影响及其机制。方法将SD雄性大鼠随机分为青年对照组(9月龄组),自然衰老组(24月龄组),TFE低、中、高剂量组,每组10只。TFE低、中、高剂量组大鼠从18月龄开始分别给予TFE 10,20,60 mg·kg^(-1)·d^(-1)灌胃给药,青年对照组和自然衰老组大鼠灌胃1%羧甲基纤维素钠(CMC-Na)。每周停药1天,持续6个月。HE染色观察心脏的组织形态,电镜观察心肌细胞中线粒体的超微结构,Western blot检测心脏组织中p21、GRP78、CHOP、Bax、P-JNK蛋白表达情况。结果 HE染色和电镜结果显示,与自然衰老组比较,TFE低、中、高剂量组心脏组织的形态结构和超微结构均有所改善。TFE还可抑制p21和GRP78蛋白的表达,降低CHOP、Bax和P-JNK蛋白的表达。结论 TFE对自然衰老大鼠心肌细胞具有保护作用。其机制可能是通过抑制内质网应激的发生,减少心肌细胞的凋亡,从而发挥对心脏的保护作用。Objective To investigate the protective effects of total flavonoids of epimedium（TFE） on myocardial cells of natural aging rats and to explore the underlying mechanisms.Methods Male Sprague-Dawley rats were randomly divided into young control group（9-month group）,natural aging group（24-month group）and TFE treatment group（10,20,60 mg·kg^-1·d^-1,orally）,with 10 individuals in each group.From the age of 18 months old,rats were treated orally with TFE（or 1 % sodium carboxymethyl cellulose）,one day for medication suspension and the treatment lasting for 6 months.The pathological changes of heart tissue were observed under light microscope after haematoxylin-eosin（HE）staining.The ultrastructure of mitochondria of myocardial cells in natural aging rats was observed under the electron microscope.Protein expression levels of p21,GRP78,CHOP,Bax,P-JNK were detected by Western blotting.Results The results showed that TFE significantly improved the morphology and ultrastructure of heart tissue in comparison with natural aging group.TFE also decreased the protein expression of p21,GRP78,CHOP,Bax and P-JNK.Conclusion TFE have protective effect on the myocardial cells of natural aging rats,and the possible mechanism is through inhibiting the occurrence of endoplasmic reticulum stress and decreasing the apoptosis of myocardial cells of natural aging rats.

关 键 词：淫羊藿总黄酮 内质网应激 心肌细胞 

分 类 号：R285.5[医药卫生—中药学]