绣球花品种遗传多样性的ISSR分析及指纹图谱构建  

Genetic diversity and fingerprint construction of varieties of Hydrangea macrophylla by ISSR markers

作　　者：彭继庆[1] 曹福祥[1] 曹基武[1] 董旭杰[1] 刘志明[1] 任锐[1] 熊亚丽[1] 张鹏鸿 

机构地区：[1]中南林业科技大学生命科学与技术学院,湖南长沙410004

出　　处：《中南林业科技大学学报》2016年第12期115-120,共6页Journal of Central South University of Forestry & Technology

基　　金：国家林业局948项目"绣球花植物新品种及繁育技术引进"(2013-4-35);湖南省科技厅重点研发项目:绣球花新优品种培育及开发利用(2016NK2143);湖南省教育厅一般项目"绣球花品种遗传多样性的ISSR研究及指纹图谱构建"(15C1433)

摘　　要：利用ISSR-PCR分子标记技术对23个绣球花品种进行遗传多样性研究,从100条引物中筛选出10条引物对供试材料基因组总DNA进行PCR扩增,共扩增出条带123条,其中多态性条带102条,占82.93%,具有较高的多态性。经Pop Gen32软件包分析,23个绣球花品种的平均有效等位基因数为1.493 7,平均Nei’s基因多样性指数为0.290 7,平均Shannon信息指数为0.435 7,遗传距离介于0~0.769 1之间,遗传一致度介于0.463 4~1.000 0之间,具有广泛的遗传多样性,梦幻蓝和史欧尼10条引物的扩增结果一致,二者可能为同一个品种。UPGMA聚类将23个品种分成2类,聚类结果与叶片特征一致。引物UBC855可以将23个绣球花品种完全区分,依此建立了23个绣球花品种的指纹图谱。研究结果为绣球花分类,品种鉴定、分子育种和子代鉴定提供了重要的依据。ISSR-PCR was used to detect the genetic diversity and relationship of 23 varieties of Hydrangea macrophylla. ISSR fingerprinting amplified by 10 ISSR primers which were selected from 100 ISSR primers revealed a total number of 123 unambiguous bands, of which 102 ones were polymorphic and the polymorphism frequency was 82.93%. As analyzed by PopGen32, the mean effective number of alleles of 23 varieties of Hydrangea macrophylla, the mean Nei＇s genetic diversity index is 0.2907, the mean Shannon information index is 0.435 7, the genetic distance between varieties ranged from 0 to 0.769 1 and the similarity coefficient ranged from 0.463 4 to 1, the genetic diversity is higher, the result of Hydrangea macrophylla ＇rathen＇ and Hydrangea macrophylla ＇masja＇ are consistent, they may be as one variety. These 23 varieties of Hydrangea macrophylla were divided into two groups by UPGMA based on the similarity coefficient, which is consistent with the classification results according to the blade features. Because 23 varieties of Hydrangea macrophylla can be completely distinguished by the UBC855 primer, the fingerprint of 23 varieties Hydrangea macrophylla was established. The result can be used as a basis for classification of Hydrangea macrophylla, variety identification, molecular breeding and offspring identify.

关 键 词：绣球花 ISSR 遗传多样性 指纹图谱 

分 类 号：S718.46[农业科学—林学]