血清COL10A1在骨关节炎中检测的意义  

Detection of serum COL10A1 level in patients with knee osteoarthritis

作　　者：马金赛 任姜栋[2] 张晓岗[2] 

机构地区：[1]新疆医科大学第一附属医院,新疆乌鲁木齐830054 [2]新疆医科大学第一附属医院关节外科,新疆乌鲁木齐840054

出　　处：《中国骨质疏松杂志》2016年第11期1370-1374,共5页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金项目资助(U1503221)

摘　　要：目的观察并探讨血清COL10A1水平在骨关节炎软骨损伤的诊断价值以及对骨性关节炎疾病活动性严重程度的预测价值。方法所收集89例KOA患者均为2014年9月至2016年4月在我院关节外科门诊就诊和住院患者,采集清晨空腹血5ml,依据影像学Kellgren-Lawrence(K-L)分级评分系统进行病情严重程度分级,Ⅰ级20例,Ⅱ级25例,Ⅲ级23例,Ⅳ级21例,正常对照组23例。各组年龄及性别构成比无显著性统计学差异,所有血清标本应用双抗体夹心酶联免疫吸附(ELISA)法检测COL10A1浓度。结果 1、KOA组患者血清COL10A1(1.19(0.61-1.93)ng/ml)浓度水平较正常对照组(0.45(0.34-0.52)ng/ml)高,且差异具有显著性统计学意义(P<0.01)。2、在骨关节炎病情预测上,血清COL10A1浓度水平在OA患者K-L分级Ⅱ级组(1.98(1.59-2.12)ng/ml)和Ⅲ级组(1.84(1.43-2.02)ng/ml)最高,且与Ⅰ级组和IV级组差异具有显著统计学意义(P<0.01),其中Ⅱ级组与Ⅲ级组患者血清COL10A1浓度水平差异不具有统计学意义(P=0.149),二者无显著差异。结论 1、骨性关节炎患者血清COL10A1浓度水平较正常对照组高。2、在骨关节炎组,血清COL10A1水平随着K-L分级等级逐渐升高,在Ⅱ级组和Ⅲ级组时达到最高,随后逐渐下降,因此具有结合影像学K-L分级预测评价患者病情严重程度的潜力。Objective To observe the relationship between the serum COL10A1 level and the severity of knee osteoarthritis（ KOA）. Methods Eighty-nine patients with KOA in our outpatient and inpatient department from September 2014 to April 2016 were selected. Five ml morning serum of each subject was collected and lateral position of X-ray detection of knee joint was performed. The X-ray images were classified according to the Kellgren-Lawrence radiographic grading criteria. All the subjects were divided into group I（ n = 20）,group II（ n = 25）,group III（ n = 23）,group IV（ n = 21）,and control group（ n = 23）. There were no significant differences of age and gender among the groups. The serum COL10A1 level was detected using enzyme-linked immunosorbent assay（ ELISA）. Re sults 1） The serum COL10A1 level in KOA group（ 1. 19 ng / ml,0. 61- 1. 93 ng / ml） was significantly higher than that in control group（ 0. 45 ng / ml,0. 34- 0. 52 ng / ml,P〈0. 01）. 2） In group KOA,the serum COL10A1 levels in group II（ 1. 98 ng / ml,1. 59- 2. 12 ng / ml） and group III（ 1. 84 ng / ml,1. 43- 2. 02 ng / ml） were the highest,and no significant difference of the serum COL10A1 level between group II and group III was observed（ P〈0. 05）. Among them,it was significantly different between group I and IV（ P〈0. 01）. Conclusions 1） The serum COL10A1 level in patients with KOA is higher than that in normal subjects. 2） In KOA group,the serum COL10A1 level increases gradually,and reaches to the highest in group II and group III,and then decreases gradually. So it has potential to evaluate the severity of the patient＇s condition combining with the K-L classification.

关 键 词：X型胶原 骨性关节炎 COL10A1 

分 类 号：R684.3[医药卫生—骨科学]