强骨饮对去卵巢大鼠骨折愈合骨小梁Micro-CT改变研究  

The effect of strengthening-bone receipt on the change of trabecular bone of fracture healing under micro-CT in rat osteoporotic model

作　　者：姚建亮[1] 王博[1] 吴鹏[1] 刘康[1] 史晓林[1] 

机构地区：[1]浙江中医药大学附属第二医院骨伤科,杭州310005

出　　处：《中国骨质疏松杂志》2016年第11期1395-1398,1403,共5页Chinese Journal of Osteoporosis

基　　金：浙江省自然科学基金(Y14H270017)

摘　　要：目的研究强骨饮对去卵巢大鼠骨折愈合骨小梁Micro-CT的影响。方法将32只雌性SD大鼠随机分为4组:A组(假手术组)、B组(模型组)、C组(强骨饮组)、D组(基础用药组)。骨松造模组(B、C、D组)术前随机取8只大鼠进行骨密度测量,后骨松造模组摘除双侧卵巢,A组切除少许脂肪后缝合,3月后随机取骨松造模组8只大鼠进行骨密度测量以证明造模成功;将所有大鼠进行骨折造模,后将C组强骨饮灌胃、D组进行碳酸钙和维生素D3灌胃,A、B两组用等量生理盐水灌胃;在6周后时各取8只大鼠股骨骨折处骨痂进行Micro-CT分析,参数包括BV/TV、Tb.Th、Tb.N、Tb.Sp。结果 B组骨小梁数量、骨小梁厚度与骨体积分数低于C、D组(P<0.05),骨小梁分离度指数高于C、D组(P<0.05);C组与D组骨小梁数量、骨小梁厚度比较差异有统计学意义(P<0.05),而与D组的骨小梁分离度及骨体积分数比较差异无统计学意义(P>0.05)。结论强骨饮能增加骨量、改善骨质疏松骨三维结构特别是增加骨小梁数量与厚度,其可能机制是强骨饮通过抑制破骨细胞活性,从而增强骨强度、促进骨折愈合。Objective To study the effect of the strengthening-bone receipt on the trabecular bone in an osteoporotic rat model of fracture healing with micro-CT. Me thods 32 female rats were randomly divided into 4 groups,group A（ sham group）,group B（ OVX group）,group C（ strengthening-bone receipt group）,and group D（ basic treatment group）. BMD was detected in 8 rats of model groups（ B,C,and D group） before surgery. The rats in model groups were then ovariectomized. Rats in group A were excised small amount of fat and sewn. Eight rats were randomly selected for BMD measurement to prove the successful model establishment in 3 months. Fracture model was conducted in all rats. Rats in group C received the strengthening-bone receipt with gavage. Rats in group D received calcium and vitamin D with gavage. Rats in group A and B received normal saline. Rats were killed in 6 weeks. The femur fracture callus was collected and the parameters including bone volume fraction（ BV / TV）,trabecular thickness（ Tb. Th）,trabecular number（ Tb. N）,and trabecular separation（ Tb. Sp） were analyzed using micro-CT. Re sults The Tb. N,Tb. Th,and BV / TV in group B were less than those in group C and D（ P〈0. 05）,but the Tb. Sp was higher than that in group C and D（ P〈0. 05）. The Tb. N and Tb. Th were significantly different between group C and group D（ P〈0. 05）,while the Tb. Sp and BV / TV between group C and group D were not significantly different（ P〉0. 05）. Conclusion The strengtheningbone receipt increases bone mass and improves three-dimensional structure of the bone,particularly Tb. N and Tb. Th. The possible mechanism of enhancing bone strength and promoting fracture healing may be through inhibiting the activity of osteoclasts.

关 键 词：中医中药 骨质疏松 强骨饮 骨折 MICRO-CT 动物实验 

分 类 号：R681[医药卫生—骨科学]