麝香对颅骨骨缺损模型大鼠基质细胞衍生因子1和肝细胞生长因子的相关性研究  

Study of the effect of musk on the relationship between stromal cell-derived factor 1 and hepatocyte growth factor in the rat model of skull bone defect

作　　者：李应福[1] 李宁[1] 谢兴文[2] 宋敏[1] 徐世红[2] 李鼎鹏 

机构地区：[1]甘肃中医药大学,兰州730000 [2]甘肃省中医院,兰州730050

出　　处：《中国骨质疏松杂志》2016年第11期1477-1480,共4页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金资助项目(81460735)

摘　　要：目的探讨麝香对颅骨骨缺损模型大鼠基质细胞衍生因子1(stromal cell-derived factor 1,SDF-1)和肝细胞生长因子(hepatocyte growth factor,HGF)水平变化的影响及其作用机制。方法 SD大鼠雌、雄各150只,利用牙科钻建立颅骨骨缺损模型,模型动物完全随机分为给药组和模型组,又将这两组分别分为3小组,每组50只。给药组灌服麝香,模型组灌服生理盐水,采用酶联免疫吸附法(ELISA)分别测定各组7d、14d、28d血清中SDF-1、HGF的变化,将所得OD值处理计算后应用SPSS17.0统计分析。结果与模型组比较,给药组第7天SDF-1含量增加,差异有统计学意义(P=0.0008),第7天和第14天HGF含量均增加(P=0.0158,P=0.0234),但第7天表达增加更加显著。结论麝香可促进大鼠颅骨骨缺损区的愈合速度,而这种愈合机制可能与增加血清中SDF-1、HGF水平有关。Objective To investigate the effect of musk on the levels of stromal cell-derived factor 1（ SDF-1） and hepatocyte growth factor（ HGF） in the rat calvarial bone defect model. Me thods 150 male and female SD rats were used to establish the model of skull bone defect with dental drill. Model animals were randomly divided into the medicine group and model group. The two groups were further divided into 3 groups,with 50 rats in each group. Rats in the treatment group received musk,and rats in the model group were gavaged with normal saline. Serum SDF-1 and HGF were determined in each group at 7 d,14 d,and 28 d with enzyme-linked immunosorbent assay（ ELISA）. The resulting OD value was analyzed using a SPSS 17. 0 software. Re sults Comparing to the model group,SDF-1 in the treatment group increased on 7 d,and the difference was statistically significant（ P =0. 0008）. HGF increased on 7 d and 14 d（ P = 0. 0234,P = 0. 0158）,and the increase was the highest on 7 d. Conclusion Musk promotes the healing of rat cranial bone defect area,and this healing mechanism may be associated with the increase of serum SDF-1 and HGF expression.

关 键 词：麝香 骨髓间充质干细胞 骨缺损 基质细胞衍生因子1 肝细胞生长因子 大鼠 研究 

分 类 号：R68[医药卫生—骨科学]