Community Structure of Endophytic Bacteria in Chestnut, Castanea mollissima  

作　　者：Chen Zebin Li Bing Xia Tiyuan Wang Dingkang Yu Lei Xu Shengguang Liu Jiani Zhang Yongfu 

机构地区：[1]College of Agriculture, Kunming University, Kunming 650214, China [2]Yunnan Engineering Research Center for Characteristic Agriculture, Kunming 650214, China [3]Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China [4]Department of Life Science and Technology,Kunming University, Kunming 650214, China

出　　处：《Plant Diseases and Pests》2016年第4期1-5,共5页植物病虫害研究（英文版）

基　　金：Supported by National Natural Science Foundation of China(31460491);Applied Basic Research Project of Science and Technology Department of Yunnan Province(2013FD040);Scientific Research Project of Education Department of Yunnan Province(2014Y390);Talent-Recruiting Program of Kunming University(YJL14005);Key Disciplines (Ecology) Project of Colleges and Universities in Yunnan Province

摘　　要：[ Objective] The paper was to detect the species of endophytic bacteria in Chestnut （Castanea mollissima）. [ Method ] The 16S rDNA-V4 region of endophytic bacteria in chestnut （sample name BL） was sequenced by Illumina MiSeq high-throughput sequencing technology. The number of sequences and op- erational taxonomic units （OTUs） for each sample was sorted and calculated using Qiime and Mothur software. The abundance and α-diversity of species were analyzed. [ Result] The number of effective sequences and OTUs were 21 167/191. The rarefaction curves showed that adequate sampling was achieved, and the number of OTUs was close to saturation. The endophytic bacteria of chestnut belonged to 10 genera, including Koribacter （3.48%） , Solibacter （2.59%） , Bra- d^hizobium （ 3. 57% ）, Rhodoplanes （ 7. 41% ）, Methylobacterium （ 4. 20% ）, Agrobacterium （ 19. 73% ）, Kaistobacter （ 2. 05% ）, Sphingomonas （18.66%）, Acidovorax （35.98%）, and Methylibium （2.32%）. The dominant species were Acidovorax, Agrobacterium and Sphingomonas. [ Conclusion] Illumina high-throughput sequencing technology provided more accurate and scientific data resources for the study of endophytic bacteria in chestnut.[ Objective] The paper was to detect the species of endophytic bacteria in Chestnut （Castanea mollissima）. [ Method ] The 16S rDNA-V4 region of endophytic bacteria in chestnut （sample name BL） was sequenced by Illumina MiSeq high-throughput sequencing technology. The number of sequences and op- erational taxonomic units （OTUs） for each sample was sorted and calculated using Qiime and Mothur software. The abundance and α-diversity of species were analyzed. [ Result] The number of effective sequences and OTUs were 21 167/191. The rarefaction curves showed that adequate sampling was achieved, and the number of OTUs was close to saturation. The endophytic bacteria of chestnut belonged to 10 genera, including Koribacter （3.48%） , Solibacter （2.59%） , Bra- d^hizobium （ 3. 57% ）, Rhodoplanes （ 7. 41% ）, Methylobacterium （ 4. 20% ）, Agrobacterium （ 19. 73% ）, Kaistobacter （ 2. 05% ）, Sphingomonas （18.66%）, Acidovorax （35.98%）, and Methylibium （2.32%）. The dominant species were Acidovorax, Agrobacterium and Sphingomonas. [ Conclusion] Illumina high-throughput sequencing technology provided more accurate and scientific data resources for the study of endophytic bacteria in chestnut.

关 键 词：High-throughput sequencing DETECTION CHESTNUT Endophytic bacteria 

分 类 号：S664.2[农业科学—果树学] Q93[农业科学—园艺学]