半枝莲提取物及其纳米颗粒对小鼠肝癌细胞H22抑制作用对比研究  

Comparative Study of Extracts and Nano-particles of Scutellariae Barbatae Herbaon Inhibitions of H22 Liver Cancer Cells in Mice*

作　　者：陈浩[1] 陈文滨[1] 程金生[1] 陈怡[1] 

机构地区：[1]惠州市第一人民医院,广东惠州516001

出　　处：《抗感染药学》2016年第5期974-977,共4页Anti-infection Pharmacy

基　　金：广东省惠州市科技计划项目(编号:20160810)

摘　　要：目的:研究半枝莲提取物(SBE)与含纳米颗粒半枝莲提取物(SBE-NP)对小鼠肝癌细胞H22的抑制作用。方法:建立小鼠肝癌细胞模型,采用称质量法检测肿瘤细胞生长的抑制程度,采用TUNEL标记法检测肿瘤细胞凋亡率,比较SBE与SBE-NP两组药物的作用效果。结果:SBE组、SBE-NP组瘤体平均质量低于空白组(P<0.01),肝癌细胞凋亡率高于空白组(P<0.01),SBE-NP组瘤体质量明显低于等剂量的SBE组(P<0.01),肝癌细胞凋亡率高于空白组(P<0.01)。结论:SBE及SBE-NP对抗肿瘤治疗均具有一定疗效,且SBE-NP的疗效优于SBE。Objective： To study the extracts and nano-particles of Scutellariae Barbatae Herba on the inhibition of H22 liver cancer cells in mice. Methods：Mouse liver cancer cell model was established and the growth inhibition of tu- mor cells was detected by weighing method. Apoptosis rate of tumor cells was detected by TUNEL labeling method, Comparison of the inhibitions of extracts of Scutellariae Barbatae Herba（SBE） and nano-partieles of Scutellariae Bar- batae Herba（SBE-NP） in the two groups of drugs. Results： SBE group and SBE-NP group were compared with the blank group and the weight of the tumor was significantly reduced（P〈0.01）. Increased apoptosis rate of hepatocellular carcinoma（P〈0.01）. SBE-NP group tumor weight was significantly lower than that of the SBE group（P〈0.01）. In- creased apoptosis rate of hepatocellular carcinoma（P〈 0.01）. Conclusion： SBE and SBE-NP in the treatment of tumors have a certain effect. The effect of SBE-NP is more prominent than SBE.

关 键 词：半枝莲提取物(SBE) SBE-NP 抑瘤率 凋亡率 

分 类 号：R965[医药卫生—药理学]