葎草多糖HSW2的分离纯化及结构初步研究  

Separation Purification and preliminary Structure Discussion of Polysaccharide HSW2 from Humulus scandens( Lour.) Merr.

作　　者：段国峰[1] 孙如宁[1] 马维坤[1] 汪晶[1] 

机构地区：[1]中国药科大学高等职业技术学院,南京211198

出　　处：《海峡药学》2016年第11期32-34,共3页Strait Pharmaceutical Journal

基　　金：中国药科大学青年教师科技基金E0425

摘　　要：目的从葎草分离纯化出多糖HSW2,研究其组成及初步结构。方法葎草经水提醇沉、脱蛋白、透析,DE-52纤维素及Sephadex G-100凝胶柱洗脱得纯化多糖HSW2;采用紫外、高效液相、气相、红外、核磁共振等色谱分析其组成及初步结构。结果 HSW2由鼠李糖、阿拉伯糖、甘露糖、葡萄糖、半乳糖组成,摩尔比为0.73∶0.99∶0.69∶0.80∶1.00分子量约为5.6×104Dal,糖环以β型吡喃糖为主,并有甲基、酰氨基、酯酰基等。结论分析了葎草多糖HSW2的初步结构,为进一步的研究打下了基础。OBJECTIVE To study the isolation,the composition and preliminary characterization of polysaccharide HSW2 from Humulus scandens （Lour. ） Merr. METHODS The crude polysaeeharides were obtained by extracting with boiling-water and precipitating with alcohol, the protein was removed by TCA, The cleared polysaccharide were purified by DE-52 cellulose, Sephadex G-100 column ,received HSW2. The composition and characterization of HSW2 was analyzed by UV, HPLC, GC, IR, 1H-NMR. RESULTS HSW2 consists of Rha, Ara, Man, Glu, Gal, in amolar ratio of 0. 73：0. 99：0. 69：0. 80： 1.00. Themolecular mass was 5.6 × 104Dal. The structure was analyzed by chromatography suggesting HSW2 was in the form of pyranose β-glycosidic bond mainly, and it contained methyl, acylamino, ester acyl group and so on. CONCLUSION The structure of HSW2 was first discussed, these work could establish foundation for the further study of the polysaccharides.

关 键 词：葎草 多糖 分离纯化 结构研究 

分 类 号：R917[医药卫生—药物分析学]