HPLC法测定烟酰胺片的含量  

Determination of Nicotinamide Tablets by HPLC

作　　者：吴凡 刘继华 王莹 

机构地区：[1]云南省食品药品检验所,昆明650011

出　　处：《海峡药学》2016年第11期74-76,共3页Strait Pharmaceutical Journal

摘　　要：目的建立HPLC法测定烟酰胺片含量的方法。方法色谱柱为月旭XB-C_(18)(250×4.6mm,5μm);以甲醇-醋酸铵(0.05mol·L^(-1)用冰醋酸调pH=6.0)(10∶90)为流动相;流速:1.0m L·min^(-1);柱温:30℃;检测波长262nm。结果烟酰胺在10.78~269.53μg·m L-1的范围内呈良好的线性关系,R^2=0.9996(n=5),重复性好,最低检测限为6ng,平均回收率为98.41%。结论本方法专属性强,准确可靠,灵敏度高,可用于测定烟酰胺的含量,有效地控制烟酰胺片的药品质量。OBJECTIVE To establish a method for the determination of nicotinamide tablets by HPLC. METHODS The chromatographic separation was performed on a Welch XB-C18 column （250 × 4. 6mm, 5μm ）, the column temperature was maintained at 30℃. ammonium acetate solution（0. 05mol . L-1, adjust by glacial acetic acid to pH 6.0） ：methanol （90： 10） as the mobile phase, the flow rate of 1.0mL . min-1. The UV detection wavelength was 262nm. RESULTS The linearity range of nicotinamide was 10. 78 - 269. 53μ g . mL-1 , R2 = 0. 9996 （ n = 5 ）, the lowest limit of detection was 6ng, the average recovery rate was 98.41%. CONCLUSION This method is simple, accurate and reliable,which can be effectively used to control the drug quality of nicotinamide tablets.

关 键 词：烟酰胺 HPLC 含量测定 

分 类 号：R927[医药卫生—药学]