HPLC法测定不同产地白毛夏枯草中木犀草素的含量  

Determination of Luteolin in Ajuga decumbens Thunb. from Different Producing Areas by HPLC

作　　者：赵丹[1] 邓祖磊 崔田[2] 葛德洲 马涛[1] 赵伟曼 来李娟 

机构地区：[1]蚌埠医学院药学系,蚌埠233000 [2]蚌埠市食品药品检验中心,蚌埠233000

出　　处：《海峡药学》2016年第11期77-79,共3页Strait Pharmaceutical Journal

基　　金：蚌埠医学院研究生科研创新计划项目(编号Byycx1554)

摘　　要：目的建立测定白毛夏枯草药材中木犀草素含量的高效液相色谱的方法,并对不同产地的白毛夏枯草药材进行含量测定。方法色谱柱为Agilent Eclipse XDB-C_(18)(4.6mm×150mm,5μm),流动相为乙腈-0.5%冰醋酸,梯度洗脱,流速为1.0m L·min^(-1),检测波长350nm,柱温30℃。结果木犀草素在20min内得到良好的分离,且进样量在0.0682~1.364μg(r=0.9999)范围内呈现良好的线性;平均回收率(n=6)为99.06%。不同批次白毛夏枯草中木犀草素的含量范围为0.0213%~0.1198%,实验结果表明,全国不同省份,不同产地白毛夏枯草药材的含量存在差异。结论该方法操作简单,灵敏度高,重现性良好,可用于白毛夏枯草的药材和饮片的质量控制。OBJECTIVE To develop an HPLC method for the determination of the contents of Luteolin in Ajuga Decumbens Thunb. from different areas. METHODS The analysis was performed on a Agilent Eclipse XDB-C18 column （4. 6mm × 150mm, 5 μm） with a mobile phrase consisted of acetonitrile （A） and 0.5 % glacial acetic acid （B） solution for gradient elution （0 -20min,20% A→50% A,80% B→50% B） at the flow rate of 1mL . min-1. The column temperature was 30℃ and the detection wavelength was 350nm. RESULTS Luteolin had good linearity in the ranges of 0. 0682 - 1. 364μg（ r =0. 9999 ） within 20 minutes and the average recoveries （ n = 6 ） was 99.06% The content ranges of Luteolin was 0. 0213% -0. 1198% for Ajuga Decumbens Thunb from different batch number. The results showed that the significant difference in samples from different provinces had been observed. CONCLUSION The method developed is simple, sensitive, reproducible, which can beapplied to quality control of Ajuga Decumbens Thunb.

关 键 词：高效液相色谱 白毛夏枯草 不同产地 木犀草素 含量测定 

分 类 号：R917[医药卫生—药物分析学]