Determination of gallic acid in the traditional Chinese medicine by high-performance capillary electrophoresis  

高效毛细管电泳法测定传统中药拳参中没食子酸含量（英文）

作　　者：高杨亚雅 朱尘琪 郭爽[1] 邱百灵 吴迪[1] 高也[1] 梁启慧[1] 韩南银[1] 张萍[2] 

机构地区：[1]北京大学医学部药学院,北京100191 [2]首都医科大学附属北京友谊医院中药剂科,北京100050

出　　处：《Journal of Chinese Pharmaceutical Sciences》2016年第10期747-753,共7页中国药学（英文版）

基　　金：supported by Beijing Kaiao Technology Development Co., Ltd., China and Beijing Friendship Hospital, Capital University of Medical Sciences Beijing

摘　　要：Rhizoma Polygoni Bistortae has been widely used as an important medicinal herb in the Far East for a few thousand years, and it has gained more recognition in the last few decades. In the present study, we used a high-performance capillary electrophoresis （HPCE） method for determination of the gallic acid （GA） in Quanshen （QS） pieces. The analysis of GA in QS pieces was successfully achieved in less than 6 min by capillary electrophoresis with UV detection at a wavelength of 290 nm. The background electrolyte was 20 mmol/L sodium borate solution （pH 9.00）. The separation was performed in an uncoated fused-silica capillary （52 cm^50 lam） with an effective length of 40 cm, and the applied voltage was 20 kV. Sample solutions were injected for l0 s at an atmospheric pressure of 5 KPa. The result showed good resolution and precision （the intra-day RSD was 0.37%）. The calibration curves of the standard solutions were linear in the range of 0.22-2.25 mg/mL, with correlation coefficients greater than 0.9990. The recovery of the method ranged from 100.6% to 107.9%, and the average recovery of GA was 105.9% with an RSD of 3.63%. HPCE is a powerful technology, and it is the first time to successfully apply such a method for the determination of GA in the traditional Chinese medicine.对拳参饮片进行质量标准研究,首次建立了采用高效毛细管电泳测定拳参饮片中没食子酸含量的方法,对拳参的质量控制很有意义。我们采用毛细管电泳法,缓冲液为pH9.0的20 mmol/L硼砂缓冲溶液;运行电压为20 KV;检测波长为290 nm;进样压力为5 KPa,进样时间10 s;运行温度为室温。检测出湖北、山东、河北3个产地7个批次的拳参饮片中没食子酸的含量:湖北2.29%,山东3.20%、6.57%,河北4.52%、2.02%、1.68%、1.76%;没食子酸在0.225–2.250 mg/mL范围内线性良好(r=0.9995);精密度RSD为0.37%(n=6);加样回收率为105.9%(RSD=3.63%,n=9)。该方法准确、可靠、重复性好,可用于拳参饮片中没食子酸含量测定。

关 键 词：Traditional Chinese medicine Quanshen Gallic acid High-performance capillary electrophoresis Quanshen pieces 

分 类 号：R284.1[医药卫生—中药学] O657.8[医药卫生—中医学]