产甲烷菌耐酸培养及其以餐厨为底物的产气特性  

Acid-tolerant cultivation of methanogen and its biogas generation characteristics from food wastes

作　　者：杨莉丽[1,2] 赵明星[1,2] 黄月[1,2] 阮文权[1,2] YANG Lili ZHAO Mingxing HUANG Yue RUAN Wenquan(School of Environment and Civil Engineering, Jiangnan University, Wuxi 214122, China Jiangsu Key Laboratory of Anaerobic Biotechnology, Jiangnan University, Wuxi 214122, China)

机构地区：[1]江南大学环境与土木工程学院,无锡214122 [2]江苏省厌氧生物技术重点实验室,无锡214122

出　　处：《环境工程学报》2016年第11期6673-6679,共7页Chinese Journal of Environmental Engineering

基　　金：江苏省科技支撑计划项目(BE2012615);江苏省自然科学基金项目(BK20130126)

摘　　要：采用逐步提高厌氧发酵体系内丁酸盐浓度的方式,对产甲烷菌进行了适应性耐酸培养,随后将培养后的污泥接种餐厨垃圾进行高固态厌氧发酵,研究其以餐厨垃圾为底物的产甲烷特性。结果表明,进行耐丁酸培养的各个组的沼气及甲烷产量、最终VFAs浓度、SCOD浓度基本和丁酸钠添加量呈正相关。其中丁酸钠添加量为8 g·L-1的组的甲烷产量和沼气产量相对稳定且较大,最终分别为229 m L和830 m L。其脱氢酶活性也相对较大,为126.74 TFμg·(m L·h)-1;培养后污泥的TS、VS较培养前提高量最大,分别提高了64.13%和67.31%,相比培养后的对照组也提高了44.05%和66.07%。而产甲烷实验研究表明,经8 g·L-1的丁酸钠培养后的污泥的产气情况最好,其累积甲烷、沼气产率及甲烷含量最大,分别为101.8 m L·g^(-1)TS、228.0 m L·g^(-1)TS和44.7%,是对照组的3.2、1.3和2.4倍。Methanogens were cultivated to tolerate acids by gradually increasing the concentration of butyrate in an anaerobic digestion system. Subsequently,food wastes were inoculated with the cultured sludge to evaluate the characteristics of methane generation by the high-solids fermentation model. The results indicate that the biogas and methane yields,and final concentrations of VFAs and SCOD in different acid-tolerant cultured groups were all positively correlated with increasing concentration of sodium butyrate. Methane and biogas yields in the group dosed with 8 g·L^（-1）sodium butyrate were relatively stable and large,with ultimate values of 229 m L and 830 m L,respectively. Dehydrogenase activity was also relatively large,at 126. 74 TF μg ·（ m L ·h）^（-1）. The TS and VS contents of the sludge after cultivation increased 64. 13% and 67. 31% respectively,compared to the initial sludge,which also increased by 44. 05% and 66. 07% compared with the cultured control group. In the methane generation experiments,maximum cumulative methane yield（ 101. 8 m L·（ g TS）^（-1））,biogas yield（ 228. 0 m L·（ g TS）^（-1））,and methane content（ 44. 7%） were achieved in the group dosed with 8g·L^（-1）sodium butyrate,representing values of 3. 2,1. 3,and 2. 4 times those of the control group.

关 键 词：餐厨垃圾 高固态发酵 丁酸 耐酸 甲烷 

分 类 号：X705[环境科学与工程—环境工程]