植物-固体菌剂联合修复石油污染土壤的基础研究  

Basic research for plant and solid bacterial agent to remediate oil-contaminated soil

作　　者：王京秀[1] 张志勇[1] 孙珊珊[1] 穆红岩[1] 万云洋[2] 赵立平[1] 李永峰[1] 张忠智[1] WANG Jingxiu ZHANG Zhiyong SUN Shanshan MU Hongyan WAN Yunyang ZHAO Liping LI Yongfeng ZHANG Zhongzhi(State Key Laboratory of Heavy Oil, China University of Petroleum, Beijing 10224-9, China State Key Laboratory of Petroleum Resource and Prospecting, China University of Petroleum, Beijing 102249, China)

机构地区：[1]中国石油大学(北京)重质油国家重点实验室,北京102249 [2]中国石油大学(北京)油气资源与探测国家重点实验室,北京102249

出　　处：《环境工程学报》2016年第11期6732-6738,共7页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金资助项目(41172333;51474223)

摘　　要：以高效石油降解菌N_2、KB为目的菌种,玉米粉、麸皮和锯末为载体制备固体菌剂,并测定了这3种载体的饱和持水量、pH值和吸菌量。以吸菌量为评价指标,结合经济性综合选择最优载体,同时还考察了温度、pH值和料水比对最优载体吸菌量的影响。通过测定微生物数量和石油降解率的变化,考察了植物微生物联合修复效果。结果表明,与玉米粉和麸皮相比,锯末饱和持水量更大、吸菌量较大。锯末是木材厂的下脚料,可以实现高价值资源化利用。在温度30℃,pH为7,料水比1∶1.5的最适培养条件下,固体菌剂中N_2和KB两种细菌的活菌数量分别高达1.00×109CFU·g^(-1)和1.58×109CFU·g^(-1)。采用生物菌剂和柳枝稷对石油污染土壤进行植物-微生物联合修复实验,100 d后石油降解率可达到50.5%。In this study,we tested corn flour,wheat bran,and sawdust as alternative solid carriers for N_2 and KB bacteria. To this end,we evaluated the influence of temperature,pH,and the material to water ratio on the capacity of the carrier to absorb bacteria. The latter and the material＇s cost were considered when choosing the optimal carrier. Moreover,plant-microbial remediation was studied by quantifying microbial abundance and oil removal rate. Results showed that saturated water content in sawdust was higher than that in either corn flour or wheat bran. Thus,sawdust was chosen as the optimal carrier because of its large bacterial uptake and origin as timber waste. Under optimal conditions of 30℃,pH 7,and a material to water ratio of 1 ∶ 1. 5,N_2 and KB solid inoculants reached 1. 00 × 109CFU·g^（-1）and 1. 58 × 109CFU·g^（-1）,respectively. The ensuing petroleum removal rate was 50. 5% after 100 days.

关 键 词：植物-微生物联合修复 高效石油降解菌 石油污染土壤 固体菌剂 锯末 

分 类 号：X53[环境科学与工程—环境工程]