ANAMMOX菌利用零价铁还原硝酸盐脱氮研究  

Biotransformation of Nitrate to Nitrogen Gas Driven by ANAMMOX Microbes via Zero-valent Iron Under Anaerobic Conditions

作　　者：周健[1,2] 完颜德卿 黄勇[1,2] 刘忻[1,2] 袁怡 李祥[1,2] 姚鹏程[1,2] 杨朋兵 薛鹏程[1] 

机构地区：[1]苏州科技大学环境科学与工程学院,苏州215009 [2]苏州科技大学环境生物技术研究所,苏州215009

出　　处：《环境科学》2016年第11期4302-4308,共7页Environmental Science

基　　金：国家自然科学基金项目(51478284;51408387);苏州科技大学科研启动项目(331411202);苏州科技大学科研基金青年项目(XKQ201414)

摘　　要：采用全混式厌氧搅拌罐,研究在自养条件下,ANAMMMOX菌利用零价铁还原硝酸盐为氮气的可行性及最佳反应条件.投加铁屑71 g·L^(-1),接种厌氧氨氧化颗粒污泥200 mg·L^(-1),控制温度33℃±0.5℃,搅拌强度150 r·min^(-1),水力停留时间10 h,pH值为7.0~8.0.在中性条件下,添加厌氧氨氧化微生物的零价铁还原硝酸盐体系一次性投加零价铁,0~5 d硝酸盐脱氮负荷达到0.12 kg·(m^3·d)^(-1).反应出水氨和亚硝酸盐始终小于2.0 mg·L^(-1),硝酸盐以氮气形式损失,出水pH较进水高值超过8.0,并且可溶性铁含量始终小于7 mg·L^(-1).硝酸盐去除氮能力始终高于0.1 kg·(m^3·d)^(-1).批试实验优化反应条件:在偏酸性条件下(pH值为4~6)反应速度加快,并且液相总氮损失率大于89%.反应温度在30~40℃时,液相总氮损失率大于89%.过于极端的pH值环境及温度环境均不利于耦合反应的进行.A novel type of nitrate removal process was investigated in this study,which coupled nitrate reduction by zero-valent iron（ ZVI） with anaerobic ammonia oxidation（ AMAMMOX）. The zero-valent iron dosage was 71 g·L^-1. The inoculation volume of ANAMMOX granular sludge was 200 mg·L^-1. The agitation rate and environment reaction temperature of the CSTR was set up to 150r·min^-1and 33℃ ± 0. 5℃,respectively. The pH of influent was maintained at 7. 0-8. 0. The result indicated that ANAMMOX bacteria could survive in nitrate and ZVI supplied system under neutral condition. The reactor was set up quickly with the nitrogen removal rate of 0. 12 kg·（ m^3·d）^-1on the first 5 days,and maintained over 0. 1 kg·（ m3·d）^-1nitrogen removal ability with the effluent nitrite and ammonia concentrations below 2. 0 mg·L^-1and pH value higher than 8. 0. The nitrogen loss was up to 89% under the condition of 30-40℃,pH 4-6. Too extreme pH and temperature conditions were not favorable for the coupling reactions.

关 键 词：厌氧氨氧化 零价铁 硝酸盐还原 耦合 氮气 

分 类 号：X703.1[环境科学与工程—环境工程]