室间隔缺损microRNA相关调控基因的初步研究  

A study of micro RNA related genes in ventricular septal defect

作　　者：许美玲[1] 吴雪嵩 朱凯[2] 李金栋[2] 王倩[2] 

机构地区：[1]泰安市泰山区疾病预防控制中心检验科,山东271000 [2]泰山医学院

出　　处：《社区医学杂志》2016年第21期1-4,共4页Journal Of Community Medicine

基　　金：山东省医药卫生科技发展计划项目(2013WS0320)

摘　　要：目的通过筛选室间隔缺损(ventricular septal defect,VSD)患者和正常对照组血清差异micro RNA(mi RNA),应用生物信息学方法预测VSD相关易感基因,为VSD的临床诊断和治疗提供理论依据。方法于2014年9月—2015年12月,采用mi RNA表达谱芯片技术在济南市儿童医院心外科取样并筛选VSD患者和正常对照组血清mi RNA差异表达情况,预测调控靶基因,经基因富集分析(gene ontology analysis,GO)和基因通路分析筛选候选基因。结果通过mi RNA芯片技术共获得36个差异表达mi RNAs,预测得到靶基因447个,新的VSD易感基因7个。文献挖掘发现这7个基因在心脏发育过程中均起着极其重要的作用。结论通过生物信息学分析发现差异mi RNA调控的7个靶基因可能与VSD密切相关,提示该病的发生是多基因相互作用的结果,为后续深化该病机制研究提供了有效的指导。Objective Screen the serum of ventricular septal defect（VSD） patients and the normal control group by micro RNA（mi RNA） microarray, and forecast VSD associated susceptibility genes by biological information, so as to provide a theoretical basis for the clinical diagnosis and treatment of VSD. Methods Mi RNAs microarray was employed to determine the differential specific expression of mi RNA, predictive control target gene and screen candidate genes by gene ontology analysis（GO） and pathway analysis from September 2014 to December 2015. Results There were 36 differential absolute expression of mi RNAs in VSD patients and 3 control subjects.A total of 447 target genes were predicted by bioinformatic methods. The GO and document mining indicated that seven new susceptibility genes were mainly related to heart development. Conclusion We found that the 7 target genes may be closely related with VSD,these suggests that the occurrence of VSD is the result of multi gene interaction, which provides effective guidance for the further study of the mechanism of the disease. These findings may reveal important insights into the pathogenesis of VSD.

关 键 词：先天性心脏病 室间隔缺损 疾病易感基因 基因富集分析 

分 类 号：R725.4[医药卫生—儿科]