环孢素A对瘢痕疙瘩成纤维细胞增殖及凋亡的影响  

Effects of Cyclosporin A on the proliferation and apoptosis of fibroblasts in keloids

作　　者：沈頔[1] 邢新[1] 杨超[1] 戴海英[1] 徐建国[1] 王晓云[1] 

机构地区：[1]第二军医大学附属长海医院整形外科,上海200433

出　　处：《中国美容整形外科杂志》2016年第11期645-647,共3页Chinese Journal of Aesthetic and Plastic Surgery

摘　　要：目的 研究环孢素A对瘢痕疙瘩成纤维细胞增殖及凋亡的影响.方法 用环孢素A作用于体外培养的瘢痕疙瘩成纤维细胞.Annexin V-FITC/PI流式细胞术检测成纤维细胞凋亡率,DAPI染色观察成纤维细胞的凋亡形态,CCK-8比色法检测环孢素A对成纤维细胞增殖的影响,并计算半数抑制浓度.结果 随着环孢素A浓度增高,成纤维细胞凋亡率不断增高,差异具有统计学意义（P＜0.05）.通过数据分析获得的半数抑制浓度为（1.908±0.280） g/L.结论 环孢素A对瘢痕疙瘩成纤维细胞的增殖具有抑制作用,并有望在瘢痕疙瘩的防治中发挥重要作用.Objective To study the biological effect of Cyclosporin A on the proliferation and apoptosis of fibroblasts in keloids. Methods Cyclosporine A was used on cultured keloid fibroblasts, then we Annexin V-FITC/PI flow eytometry, DAPI staining and CCK-8 colorimetrie were used to detect the rate of fibroblast ap- optosis, the apoptotic fibroblasts form and the half inhibitory concentration, respectively. Results With the in- creased concentration of Cyclosporin A, the apoptosis rate of fibroblasts constantly increased with a statistically significant difference. Data analysis was obtained by the half inhibitory concentration （ 1. 908±0. 280） g/L. Conclusion Cyclosporin A inhibits the proliferation of fibroblasts in keloids and is expected to play an important role in the prevention of keloids.

关 键 词：瘢痕疙瘩 环孢素A 成纤维细胞 细胞凋亡 细胞增殖 

分 类 号：R619.6[医药卫生—外科学]