A novel Arabidopsis miRNA, ath-miR38-3P, is involved in response to Sclerotinia sclerotiorum infection  

作　　者：ZHAO Xu SHAN Ya-nan ZHAO Yan WANG Ai-rong WANG Zong-hua 

机构地区：[1]Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens,Fujian Agriculture and Forestry University [2]Key Laboratory of Bio-Pesticide and Chemistry Biology,Ministry of Education

出　　处：《Journal of Integrative Agriculture》2016年第11期2556-2562,共7页农业科学学报（英文版）

基　　金：supported by grants from the Special Fund for Agro-scientific Research in the Public Interest,China(201103016);the Fujian Provincial Science Foundation,China(2013J06007)

摘　　要：Plant defense responses against penetration or colonization of pathogens are mediated by activation and repression of a large array of genes. Host endogenous small RNAs are essential in gene expression reprogramming process. We identified a new Arabidopsis micro RNA（mi RNA） ath-mi R38-3P by high-throughput sequencing and further confirmed it by Northern blot assay. Interestingly, ath-miR38-3P was highly induced after infection of the pathogen Sclerotinia sclerotiorum. Further analysis based on the mi RNA target database demonstrated that ath-mi R38-3P might target to five putative genes： AT2G03140, AT5G59430, AT5G66320, AT1G36620 and AT3G03820. To confirm the target, we conducted the quantitative real-time PCR to observe the expression pattern of each candidate gene. The results showed that only AT3G03820 was down-regulated after inoculation of S. sclerotiorum. In addition, overexpression of ath-miR38-3P down-regulates AT3G03820, suggesting AT3G03820 might represent the target for ath-mi R38-3P. Our results may provide the useful information for further studying the biological function of a novel ath-mi R38-3P and its targets in Arabidopsis-Sclerotinia interaction.Plant defense responses against penetration or colonization of pathogens are mediated by activation and repression of a large array of genes. Host endogenous small RNAs are essential in gene expression reprogramming process. We identified a new Arabidopsis micro RNA（mi RNA） ath-mi R38-3P by high-throughput sequencing and further confirmed it by Northern blot assay. Interestingly, ath-miR38-3P was highly induced after infection of the pathogen Sclerotinia sclerotiorum. Further analysis based on the mi RNA target database demonstrated that ath-mi R38-3P might target to five putative genes： AT2G03140, AT5G59430, AT5G66320, AT1G36620 and AT3G03820. To confirm the target, we conducted the quantitative real-time PCR to observe the expression pattern of each candidate gene. The results showed that only AT3G03820 was down-regulated after inoculation of S. sclerotiorum. In addition, overexpression of ath-miR38-3P down-regulates AT3G03820, suggesting AT3G03820 might represent the target for ath-mi R38-3P. Our results may provide the useful information for further studying the biological function of a novel ath-mi R38-3P and its targets in Arabidopsis-Sclerotinia interaction.

关 键 词：MIRNA Arabidopsis thaliana Sclerotinia sclerotiorum miRNA-target gene 

分 类 号：S432.4[农业科学—植物病理学]