运动与白藜芦醇对老年肥胖大鼠内脏脂肪抵抗素mRNA表达及血浆抵抗素浓度的影响  

Effects of exercise and resveratrol on visceral fat resistin expression and plasma resistin concentration in elderly obese rats

作　　者：吴琳[1] 柏友萍[1] 林潺 张皓月[1] 陈晨[1] 崔嘉琴 

机构地区：[1]安徽师范大学体育学院,芜湖241003

出　　处：《卫生研究》2016年第6期951-956,962,共7页Journal of Hygiene Research

基　　金：安徽师范大学研究生科研创新与实践重点项目(No.2015cxsj011zd)

摘　　要：目的探讨运动与白藜芦醇对老年肥胖大鼠内脏脂肪抵抗素表达及血浆抵抗素浓度的影响。方法清洁级雄性21日龄SD幼鼠分生长发育期、壮年期和老年期三个阶段增肥,建立老年肥胖大鼠模型,选取6只自然生长老年大鼠为空白对照组（C）,选取24只肥胖老年大鼠为实验组,随机分为4组,每组6只,分别为肥胖对照组（OC）,补充白藜芦醇组（OR）,运动组（OE）,运动结合白藜芦醇组（OER）。动物跑台速度及时间为每组15 m/min×15 min,4组/次,组间休息5 min,60 min/次;白藜芦醇灌胃52.5 mg/kg,1次/d;5次/周,连续8周。测血糖、血胰岛素,计算胰岛素抵抗,检测内脏脂肪组织抵抗素mRNA表达及血浆抵抗素浓度。结果 OC组的体重、内脏脂肪重量和体脂率、血糖、胰岛素抵抗均高于C组（P〈0.01,P〈0.05）,内脏脂肪组织抵抗素mRNA表达×10~3（29.80±4.91）和血浆抵抗素浓度[（4.30±1.93）μg/L]高于C组[分别为（23.09±6.12）和（3.49±0.42）μg/L],但差异无统计学意义。OE、OER组体重、内脏脂肪重量和体脂率、血糖、胰岛素抵抗低于OC组（P〈0.01,P〈0.05）,OR组低于OC组,OER组低于OE组,但差异无统计学意义。OR组内脏脂肪组织抵抗素mRNA表达（×10~3）（21.63±4.91）低于OC组（P〈0.05）,OE、OER组[分别为（28.21±4.80）、（25.22±11.12）]低于OC组,但差异无统计学意义。OR、OE和OER组血浆抵抗素浓度[分别为（3.71±1.01）、（3.29±1.19）和（2.99±0.44）μg/L]低于OC组,OER组低于OE组,OE组低于OR组,但差异无统计学意义。结论老年肥胖伴随胰岛素抵抗和高血糖,内脏脂肪抵抗素表达及血浆抵抗素浓度偏高;单纯运动及运动结合白藜芦醇明显降低老年肥胖大鼠的体重、内脏脂肪重量、体脂率、血糖和胰岛素抵抗,但对内脏脂肪抵抗素表达及血浆抵抗素浓度影响较小,单纯补充白藜芦醇能降低内脏脂肪组织抵抗素mRNA表达。Objective To exploer effects of exercise and resveratrol on visceral fat resistin expression and plasma resistin concentration in elderly obese rats.Methods SD rats as three stage of fattening divided the growth period,middle-aged and elderly,toestablish rats model of elderly obesity.6 natural growing aged rats were selected as blank control group（ C）.24 elderly obese rats were randomly divided into 4 groups,6 rats in each group： obese control group（ OC）,supplementary resveratrol group（ OR）,exercise group（ OE）,exercise combined with resveratrol group（ OER）.Running speed and time of animal were 15 m/min×15 min for each group,4 groups/times,rest between groups5 min,60min/time.For resveratrol treatment,each rat was fed 52.5mg/kg,one time/day,5 times/week,for 8 weeks.The content of blood glucose was measured,the insulin resistance was calculated,testing the expression of resistin mRNA in visceral adipose tissue and plasma Resistin concentration.Results Body weight,visceral fat mass,body fat ratio,and blood glucose,and insulin resistance of OC group were higher than C group（ P 〈 0.01,P 〈 0.05）,Resistin mRNA×10~3 expression in visceral adipose tissue and plasma concentration of OC group（（ 29.80 ± 4.91） μg/L and（ 4.30 ± 1.93） μg/L,repectively） were higher than C group（（ 23.09 ± 6.12） μg/L and（ 3.49 ± 0.42） μg/L,repectively）,but the difference was not statistically significant.Body weight,visceral fat mass,body fat rate,and blood glucose,and insulin resistance of OE group and OER group were lower than OC group（ P 〈 0.01,P 〈 0.05）,of OR group were lower than OC group,of OER group were lower than OE group,but the difference was not statistically significant.Resistin mRNA×10~3 expression in visceral adipose tissue of OR group（ 21.63 ±4.91） was lower than OC group（ P 〈 0.05）,of OE group and OER group（（ 28.21 ± 4.80）,（ 25.22 ± 11.12）,respectively） were lower than OC group,but the difference was not statistically significant.Plasm

关 键 词：运动 白藜芦醇 老年肥胖 内脏脂肪 抵抗素 

分 类 号：R589.2[医药卫生—内分泌] R455[医药卫生—内科学]